Examples of 'molecular beacons' in a sentence
Meaning of "molecular beacons"
molecular beacons ~ a term from molecular biology referring to specific probes that can bind to complementary DNA sequences. They are often used in techniques like real-time PCR for detecting and measuring gene expression
How to use "molecular beacons" in a sentence
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molecular beacons
The structure of molecular beacons is as follows.
Molecular beacons may be used in a variety of assay systems.
Principle of molecular beacons.
Molecular beacons technology is described more fully below.
The structure and working mechanism of molecular beacons is well known in the art.
Molecular beacons can be used in a variety of assay systems.
Dendrimers have also been conjugated to fluorochromes or molecular beacons and shown to enter cells.
Molecular beacons act in a similar manner to TaqMan probes.
Principle of live cell based assays using molecular beacons and beacon barcoded constructs.
Molecular beacons do not fluoresce when they are free in solution.
The kit disclosed herein comprises molecular beacons having the following nucleotide sequences.
Molecular beacons are extraordinarily specific and can discern a single nucleotide polymorphism.
Thereafter each of the amplicons can be fluorescently labelled using molecular beacons or the like.
Molecular beacons are oligonucleotide hairpins which undergo a conformational change upon binding to a perfectly matched template.
Isolating the cells that exhibit fluorescence of each of the first and second molecular beacons.
See also
For both molecular beacons the underlined sequence represented the complementary ends of the molecular beacon.
All the oligonucleotides and the molecular beacons purchased were HPLC purified.
Molecular Beacons provide a particularly useful approach.
Probe Check results for intact and partially intact Molecular Beacons probes.
Molecular Beacons have improved polymorphism discriminating capabilities.
Examples of suitable molecular beacons are,.
Molecular Beacons have been described for use in sequence detection.
In a particular embodiment of the method, the probes are constituted by molecular beacons.
It has been shown that Molecular Beacons targeted to RNAs function in fixed cells.
In other embodiments, the probes utilized in the disclosed methods are molecular beacons.
Different colors of molecular beacons are synthesized for detection of HCV or HIV.
Hydrolysis probes include TaqMan probes, molecular beacons and scorpions.
Nucleic acid Molecular Beacons are susceptible to degradation by endonucleases and exonucleases.
Accordingly, hairpin probes function similar to molecular beacons in some of these embodiments.
The molecular beacons can not efficiently detect the variant sequences of DNA or RNA targets.
Protease-triggered photodynamic molecular beacons for cancer imaging and treatment.
The molecular beacons were added to a final concentration of 3µM.
In an alternative homogeneous hybridization based procedure, molecular beacons are used for allele discriminations.
Molecular beacons are highly sequence-specific.
The markers used in the probes according to the invention and the molecular beacons are,.
Thus, molecular beacons have a fluorophore and a quencher at opposite ends.
Examples of probes that can self-anneal include molecular beacons and scorpion probes.
Molecular beacons are single-stranded oligonucleotides having a stem-loop structure.
The transfected cells were incubated with the molecular beacons alone for at least 2 hours.
Molecular beacons can be used as amplicon detector probes / primers in diagnostic assays.
In this assay, the genomic DNA is amplified in the presence of both molecular beacons.
For instance, molecular beacons typically comprise a fluorophore and a quenching agent.
CPT can be used in conjunction with other technologies, like molecular beacons and qPCR.
Thus, molecular beacons may advantageously be employed in certain embodiments of the invention.
DNA and RNA aptamers include, but are not limited to, primers and molecular beacons.
Yet another technique termed molecular beacons makes use of synthetic fluorescein-labeled oligonucleotides.
In one example of a hybridization-based method, molecular beacons can be used.
In preferred embodiments, molecular beacons are used in post-amplification detection of the target nucleic acids.
The method of claim 2 or 3, wherein the first and second molecular beacons comprise different fluorophores.
Molecular beacons are single stranded nucleic acid molecules with a stem-and-loop structure.
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