Examples of 'multiple cloning site' in a sentence
Meaning of "multiple cloning site"
In molecular biology, a multiple cloning site (MCS) is a short segment of DNA which contains many restriction endonuclease cleavage sites, typically 6 base pairs long. It is used in the process of recombinant DNA technology to insert foreign DNA into a plasmid vector
How to use "multiple cloning site" in a sentence
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multiple cloning site
A new multiple cloning site is introduced in pCIneo.
These genes are introduced to the multiple cloning site.
A new multiple cloning site is introduced in pClneo.
A selectable marker optionally linked to a multiple cloning site.
Sequence of the multiple cloning site of pFSgpt.
Promoter and terminator are separated by a multiple cloning site.
The multiple cloning site was also verified by sequencing.
The vector may comprise a multiple cloning site.
A multiple cloning site precedes the truncated phoA gene.
The vector preferably contains a multiple cloning site.
The multiple cloning site is another element of the shuttle vector.
These genes were introduced into the multiple cloning site.
Contain a multiple cloning site.
Multiple cloning site.
Creating a multiple cloning site.
See also
In addition bla gene was cloned into the multiple cloning site.
The two strands of the multiple cloning site are synthesized de novo.
The vector of the invention may comprise a multiple cloning site.
The next step removed the multiple cloning site from the phaseolin promoter.
The vector of the disclosure may comprise a multiple cloning site.
Cloning into the multiple cloning site allows for expression under control of the trc promoter.
A further useful element in an expression vector is a multiple cloning site or polylinker.
The multiple cloning site and lacZ gene were removed using Pvu II.
Preferred vectors will usually comprise at least one multiple cloning site.
The sequence of the new multiple cloning site is as follows see Seq.
A major modification resulted from the insertion of a specifically designed multiple cloning site.
This creates the multiple cloning site MCSII for insertion of STAR elements.
The complete expression cassettes were cloned in a multiple cloning site of the pBINPLUS.
A multiple cloning site was obtained by hybridization of the following 2 synthetic oligonucleotides,.
A diagram showing the process of inserting a multiple cloning site into a plasmid vector.
A multiple cloning site for insertion of the gene to be expressed ; and.
Preferred vectors and plasmids are constructed with at least one multiple cloning site.
Sequence of the multiple cloning site of the insertion plasmid pP2mxgpt.
In one embodiment of the present invention the vector comprises a multiple cloning site.
More preferably, the multiple cloning site is further operably linked to a promoter.
The mACT cDNA was then cloned into pMHint using the multiple cloning site.
A multiple cloning site having at least two restriction sites unique to said plasmid ;.
A Bam HI linker was inserted into the Hind III site of the multiple cloning site.
Preferably, the vector includes a multiple cloning site between said identical promoters.
The multiple cloning site in g1ZD was then used to insert transgene coding sequences.
The TCR-expressing sequences were cloned into multiple cloning site of pMIG plasmid.
Downstream of the multiple cloning site is the dual rrnB T1 T2 transcription terminator.
Not all unique restriction enzyme sites in the multiple cloning site of pIG3B are indicated.
The multiple cloning site shown by the triangular segment is part of pUC18.
The restriction sites within and outside the multiple cloning site of pOK12 are indicated.
It carries a 54 bp multiple cloning site with recognition sites for 13 different restriction endonucleases.
Optionally, the vectors may include a promoter region upstream from the multiple cloning site.
A pUC19 cloning vector showing the multiple cloning site sequence with restriction enzyme sites.
It is added to a vector to create a novel polylinker or multiple cloning site ( MCS ).
It contains the multiple cloning site of plasmid pUC18.
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