Examples of 'n-terminal methionine' in a sentence
Meaning of "n-terminal methionine"
N-terminal methionine: Refers to the methionine amino acid residue that is at the beginning (N-terminus) of a protein or peptide chain. It is often removed post-translationally in a process called N-terminal methionine excision
How to use "n-terminal methionine" in a sentence
Basic
Advanced
n-terminal methionine
N-terminal methionine is bacterial expression system artefact.
In the cDNAs so obtained the N-terminal methionine was not included.
The N-terminal methionine is present primarily as a convenience for bacterial expression.
In all cases of bacterial expression, an N-terminal methionine residues is added.
An additional N-terminal methionine is present in the final polypeptide.
The receptor or variant thereof may include an N-terminal methionine.
The engineered N-terminal methionine residue is not shown.
Open reading frames are usually shown with a N-terminal methionine.
Additionally, the N-terminal methionine in these variants was replaced by an acetyl group.
Other hosts require in vitro removal of the N-terminal methionine.
The N-terminal methionine can be present primarily as a convenience for bacterial expression.
The composition was in agreement with that of natural IL2 having a supplementary N-terminal methionine.
The N-terminal methionine may optionally be cleaved from the expressed polypeptide.
As noted above, the polypeptides also may have the additional N-terminal methionine.
The N-terminal methionine is usually deformylated as the nascent peptide chain is elongated.
See also
Furthermore, bacterial host cells generally introduce an additional N-terminal methionine to the products.
The N-terminal methionine derived from the prokaryotic translational start signal is put in parenthesis.
A functional open reading frame requires a start codon encoding an N-terminal methionine residue.
The predicted N-terminal methionine of the mature protein and the transmembrane region are underlined.
In particular embodiments, the protein is encoded by a nucleic acid that encodes an N-terminal methionine.
The truncated form with an additional N-terminal methionine was expressed in E. coli.
In one specific embodiment, a protein encoded by a polynucleotide of the invention lacks the N-terminal methionine.
In all cases of bacterial expression, an N-terminal methionine residue is added.
Furthermore, depending on the adjacent amino acids, a methionyl amino peptidase often removes the N-terminal methionine.
In many cases, however, the N-terminal methionine residues is cleaved off post-translationally.
Alternatively, in a bacterial expression system, the recombinant polypeptide may include a N-terminal methionine residue.
The N-terminal methionine was removed from derivatives of human interleukin-2 and human growth hormone.
The present invention is accordingly concerned with polypeptides with or without a N-terminal methionine residue.
Wherein an N-terminal methionine as set forth in SEQ ID NO, 2 is optional.
The pharmaceutical composition according to claim 2 wherein the polypeptide lacks the N-terminal methionine residue.
According to the invention, the N-terminal Methionine of a T7 variant can be replaced by a His-tag.
Depending upon the host expression system, the polypeptide may include an N-terminal methionine residue.
The pharmaceutical composition of claim 3, wherein the N-terminal methionine is present in the G-CSF polypeptide.
Therefore, many researchers have attempted to produce natural type HGH without the N-terminal methionine.
It is not yet known if the N-terminal methionine ( met1 ) is present.
However, the invention claimed herein also includes the polypeptides without the additional N-terminal methionine.
Thermostability of TaqA288 with and without N-terminal methionine was indistinguishable.
The synthetic linker utilized E . coli codons and provided for a N-terminal methionine.
The present invention provides a method for specific removal of the N-terminal methionine from methionylpeptide.
The synthetic linker duplex utilized E. coli codons and provided for an N-terminal methionine.
The polypeptide of claim 8 having an N-terminal methionine residue.
The synthetic linker duplex utilized optimal E. coli codons and encoded an N-terminal methionine.
Alternatively, the His-tag can be appended to the N-terminal Methionine of the T7 variant.
But the present invention also encompasses the T7 variants which do not include N-terminal Methionine.
Detecting MetAP2 inhibition by determining EEF1A with N-terminal methionine residue ( MetEEF1A ).
A protein encoded by a polynucleotide of any one of claims 1 to 3 lacking the N-terminal methionine.
Such cells typically produce proteins comprising a N-terminal Methionine residue.
In another embodiment, the arginase is Arginase I lacking an N-terminal methionine.
The G-CSF polypeptide for the use of claim 1, wherein the N-terminal methionine is present.
The method of claim 1, wherein said fusion protein comprises an N-terminal methionine.
You'll also be interested in:
Examples of using N-terminal
Show more
N-terminal processing of proteins exported by malaria parasites
Single chain binding molecules comprising n-terminal abp
N-terminal sequencing was then performed as described above
Examples of using Methionine
Show more
For the determination of methionine in the additives
Methionine is used in during the rest translation
Amount of the hydroxy analogue of methionine added
