Examples of 'ncoi' in a sentence
Meaning of "ncoi"
NCOI stands for 'non-commissioned officer in charge' and is used in military contexts to denote a lower-ranking officer responsible for certain duties
How to use "ncoi" in a sentence
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ncoi
The NcoI site is in lower case letters.
The orientation is tested by restriction with NcoI.
The position of the NcoI site is indicated by an asterisk.
All five had the NcoI site.
The HindIII and the NcoI restriction sites are in italics.
The vector was digested with HindIII and NcoI.
The position of the engineered NcoI site is indicated.
The NcoI site is underlined.
The biotin tags can be cut off by NcoI digest.
The NcoI and AflIII sites are lost as a result of the cloning.
In preferred embodiments the restriction enzymes are NcoI and NotI.
Screened colonies using ApaI and NcoI to identify clones with inserts.
The new plasmid was next cleaved with BglII and NcoI.
For BglII and NcoI only sites relevant for the construction are included.
This adapter fragment was cleaved by the enzymes NcoI and BglII.
See also
The new NcoI does not influence the amino acid sequence of tau protein.
Presence of the additional restriction site was also confirmed by NcoI digestion.
The NcoI site would include the codon for the initiator methionine.
This fragment was isolated and digested with XhoI and NcoI.
The NcoI site was introduced to facilitate cloning straight after the signal peptide.
These DNAs also have the cohesive ends of HindIII and NcoI.
Restriction site StuI preceded NcoI site which overlapped the initiation codon.
The resultant amplified fragment was recovered and cleaved with NcoI and BamHI.
Assembly PCR was digested with NcoI and BamHI restriction endonucleases.
Such pairs would have protruding ends compatible with ligation to NcoI at one end.
It incorporates both BamHI and NcoI restriction sites and an ATG start codon.
The beads were washed and resuspended in buffer and a restriction digest with NcoI was performed.
The NcoI site of this fragment was filled in with Taq polymerase and dNTPs.
Five of the clones contained the correct construct and were further analyzed by NcoI digestion.
The NcoI site encodes a Kozak sequence for efficient translation of the protein products.
The remaining sequence encodes a restriction site for NcoI and a cleavage site for thrombin.
The PCR product was cleaned and digested with NcoI.
PCR products were digested with NcoI restriction enzyme.
The IRES sequence was excised by digestion with the restriction enzymes BamHI and NcoI.
An alternative vector is constructed which utilizes an NcoI site at the initiating ATG.
The cAb-Lys3 containing fragment is further cleaved with an additional digestion with NcoI.
Targets are created by inserting a promoter at the NcoI site upstream of the first FRT.
The vector pAlLo2 was linearized by digestion with NcoI.
The vector pAGA3 was also digested with NcoI and XbaI restriction enzymes.
Before ligation into the vector, these double strand oligonucleotides were first digested with PstI + NcoI.
In constructing pBY1, the filled NcoI site provides an ATG start for translation initiation.
The preparation was digested with NcoI and Xhol.
The new NcoI site also changed codon two of NMT1 from a serine to an alanine.
The isolated plasmid DNA was digested with NcoI and BamHI.
NcoI single sites are commonly found in initiation codon of E. coli expression vectors e . g.
DNA is isolated from transformants and analysed by restriction with NcoI and HindIII.
Previously linearised with NcoI and treated with S1 nuclease and alkaline phosphatase.
Enzymes that cut within the HAP cDNA include NcoI and EcoRI.
The restriction enzyme NcoI has a recognition sequence ( CCATGG ) which incorporates the nucleotides ATG.
Plasmid DNA was isolated and screened by restriction analysis using NcoI and HindIII.
