Examples of 'ngr' in a sentence
Meaning of "ngr"
NGR stands for Natural Gasoline Range and is used to describe a certain range of hydrocarbons found in natural gas
How to use "ngr" in a sentence
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ngr
Bound NgR is then detected by methods well known in the art.
The peptide test compounds are contacted with NgR and washed.
The polymer can be conjugated to the NgR polypeptide using conventional chemistry.
Automated sequencing methods can be used to obtain or verify the nucleotide sequence of NgR.
The resulting mutants can be screened for NgR biological activity.
Those NgR fusión proteins of the present invention are set out in the appendant claims.
The disclosure also teaches methods of modulating NgR expression or activity for therapeutic purposes.
Also provided by this disclosure are antisense polynucleotides that recognize and hybridize to NgR polynucleotides.
The disclosure teaches gene therapy to restore NgR activity to treat those disease states.
These results demonstrate that stable cell lines were established with reduced NgR expression.
Examples of such inhibitory agents include antisense NgR nucleic acid molecules and anti NgR antibodies.
NgR is implicated in neuronal plasticity and regeneration.
Antisense polynucleotides are particularly relevant to regulating expression ofNgR by those cells expressing NgR mRNA.
PNAs of NgR can be used in therapeutic and diagnostic applications.
Such antibodies are useful in binding Nogo to prevent Nogo signaling through the NgR.
See also
NgR polypeptides of the invention can be fused to a polypeptide tag.
The exact mechanism through which MAG inhibits neuroregeneration appears to be through binding of NgR.
The ratio of NgR vs GAPDH was used to measure NgR expression levels.
Preferably, the cell produces little or no endogenous NgR polypeptide.
NgR mRNA transcripts have been found in the brain and heart.
Example NgR domains Domain.
NgR and GAPDH signals on western blot results were quantified by densitometry scanning.
In other instances, the NgR expression vector is a yeast expression vector.
Alternatively, mutations can be introduced randomly along a NgR coding sequence.
DNA vaccine against NgR promotes functional recovery after spinal cord injury in adult rats.
Yet another biologically active portion of a NgR protein of this disclosure, e . g.
The NgR domain designations used herein are defined as follows,.
Thus, novel ligands based on the predicted structure of NgR can be designed.
The NgR polypeptides of the invention, in certain embodiments, are soluble polypeptides.
In still another aspect, the disclosure provides substitution variants of NgR polypeptides.
Alternatively, NgR can be expressed in insect cells using baculovirus expression vectors.
In another instance, the agent inhibits one or more NgR protein activity.
Optionally, NgR may be purified from the cells using standard chromatographic techniques.
In one instance, the agent stimulates one or more NgR protein activity.
For example, NgR receptor activation may mediate the prevention of neurite outgrowth.
A host cell of the invention, in a suitable medium such that NgR protein is produced.
Once solubilized, NgR polypeptide can be analyzed using gel electrophoresis, immunoprecipitation or the like.
For instance, a mutein may have an increased or decreased neuron or NgR binding activity.
Optionally, the NgR polypeptide is conjugated to the polyethylene-glycol moiety through a labile bond.
Antibodies that recognize and bind fragments of the NgR polypeptides of this disclosure, e . g.
A NgR protein of the present invention, may contain at least four of the above-identified domains.
The disclosure also provides cell-based assays to identify binding partner compounds of a NgR polypeptide.
An alignment of all the NgR proteins is shown in Figure 9.
Figure 23 shows four NeuroScreen cell clones that were established with different levels of NgR knockdown.
In another instance, NgR proteins are produced by recombinant DNA techniques.
Cell-based HTS systems are contemplated to investigate NgR receptor-ligand interaction.
NgR-neutralizing antibodies comprise one class of therapeutics useful as NgR antagonists.
In preferred instances, the NgR is a human NgR and the Fc is also human.
NgR-immunoglobulin fusión proteins can be used to affect the bioavailability of a NgR cognate ligand.
This disclosure further embraces other species, preferably mammalian, homologs of the human NgR DNA.
