Examples of 'non-complementary' in a sentence
Meaning of "non-complementary"
Non-complementary is an adjective used to describe things that do not go well together or do not complete each other
How to use "non-complementary" in a sentence
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non-complementary
Non-complementary domains may also be combined.
No hybridization with non-complementary oligonucleotide was performed.
Adaptors can comprise a complementary portion and a non-complementary portion.
A non-complementary nucleotide is defined herein as a mismatch.
This served as a control for non-complementary binding.
A stretch of non-complementary nucleotide residues can be interspersed.
Negligible signal changes were observed with completely non-complementary sequences.
Typical non-complementary sequences comprise restriction sites or promoter sequences.
This sequence is referred to as the non-complementary portion of the primer.
Such non-complementary fragments typically code for an endonuclease restriction site.
Each hairpin adaptor comprises a random sequence on its non-complementary portion.
The presence of a non-complementary region has been found to enhance strand displacement.
The looped region of the second oligonucleotide probe represents a non-complementary region.
Alternatively, the non-complementary nucleobase may be at an internal position in the antisense oligonucleotide.
The analogous oligonucleotide may contain a non-complementary region, such as a promoter region.
See also
The non-complementary nucleotide sequences of the primers may include restriction enzyme sites.
A joiner oligo can be used to join non-complementary ends of two nucleic acids.
In one embodiment the duplex tags are polynucleotides having complementary and non-complementary portions.
The wall contour profile can be non-complementary to the face contour profile.
One unique feature of this approach is that bisulfite conversion creates two non-complementary strands.
The linkage may be a non-complementary region, or a phosphodiester linkage with or without modification.
The stem regions of the three arms may further comprise non-complementary regions.
In one embodiment, a non-complementary nucleobase is located in the wing segment of a gapmer antisense oligonucleotide.
In certain embodiments, the second tag section is non-complementary to the target nucleotide sequence.
Such non-complementary polynucleotides might code for an endonuclease restriction site or a site for protein binding.
In some methods, the spacer of the joiner oligo comprises non-complementary nucleic acids.
The nature and function of these non-complementary sequences will be described in detail hereafter.
The second probe further comprises a tag section that is essentially non-complementary to the target section.
Bubble shaped adapters have non-complementary sequences flanked on both sides by complementary sequences.
When complementarity is not preserved, it is preferred that the non-complementary base pairs be TG.
In one embodiment, at least one non-complementary region may be designed to form a loop region.
To avoid such problems, the IBR must be short enough to prevent non-complementary binding.
Both members of at least one pair of non-complementary strands within the complex have labels.
The HNH domain cleaves the complementary strand, while the RuvC domain cleaves the non-complementary strand.
In this case non-complementary overhangs are created corresponding to adjacent stretches of DNA.
The negative control is conducted with the target 3a non-complementary.
Non-complementary domains may also be combined, e.
The 5 ' region of the primer may be non-complementary to the target nucleic acid.
When the non-complementary oligonucleotide is used, no fluorescence is detected.
The template-complementary oligonucleotide may contain a non-complementary region such as a 5 ' promoter region.
Essentially non-complementary to said target nucleic acid ; and.
However, even when a promoter sequence is not present, other non-complementary nucleotide fragments may be attached.
These target, non-complementary sequences may have other functions.
Actin and p53 targets showed little cross-hybridization to non-complementary primer probes tethered to the surface.
Any non-complementary nucleotide sequences are preferably not at the 3 ' end of the primer.
In addition, the downstream primer contains T7 RNA polymerase promoter sequences and the non-complementary sequences.
Generally speaking, the non-complementary sequence does not code for a protein.
These 1, 2, or 3 nucleotides of the sense strand are referred to as non-complementary nucleotides.
Any such non-complementary sequences in the primer are preferably 5 ′ to the complementary sequences.
Preferably, the primers contain a non-complementary 5 ' region.
