Examples of 'o-phenylenediamine' in a sentence
Meaning of "o-phenylenediamine"
o-phenylenediamine (noun) - A chemical compound commonly used in hair dyes and hair coloring products
How to use "o-phenylenediamine" in a sentence
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o-phenylenediamine
The color was developed with o-phenylenediamine solution.
A solution of o-phenylenediamine is then added and color formation is detected using suitable plate readers.
Antibodies were then revealed by adding o-phenylenediamine as a substrate.
Then a solution of o-phenylenediamine is added and color formation is detected with a suitable ELISA reader.
Color reaction is performed with o-phenylenediamine.
Chromophores may be o-phenylenediamine or similar compounds.
It is mainly used as a precursor to o-phenylenediamine.
Condensation of this tripyrrane with O-phenylenediamine constitutes the second critical step in the synthetic pathway.
Thereafter the peroxidase is determined with a substrate, such as o-phenylenediamine.
One hundred microliters of an o-phenylenediamine solution is added to each well.
Most preferably, the detecting agent comprises hydrogen peroxide and o-phenylenediamine.
The o-phenylenediamine compound was dissolved in acetonitrile / water.
The horseradish peroxidase enzymatic activity was detected with o-phenylenediamine dihydrochloride.
In particular, o-phenylenediamine is even more preferably used.
Horseradish peroxidase enzymatic activity was quantified with o-phenylenediamine which produces a colorimetric signal.
See also
O-phenylenediamine and its salts.
The filtrates were then concentrated to afford the desired substituted o-phenylenediamine in near-quantitative yields.
The o-phenylenediamine dihydrochloride tablets were purchased from the company Dako ( Glostrup, Denmark ).
The plates were developed using the Biomerieux color kit containing o-phenylenediamine and hydrogen peroxide.
It is an isomer of o-phenylenediamine and p-phenylenediamine.
The wells were then emptied, washed with PBST and substrated buffer containing o-phenylenediamine added.
Suitable surfactants include, for example, o-phenylenediamine or lignosol compounds such as calcium lignosulphonate.
The method of claim 10, wherein the detecting agent comprises hydrogen peroxide and o-phenylenediamine.
Bound secondary antibody was quantified using SIGMA o-phenylenediamine dihydrochloride tablets Cat . No.
O-phenylenediamine 2 HCl ( OPD ) and hydrogen peroxide were added for HRP color development.
The horseradish peroxidase was then quantified with o-phenylenediamine by reading the absorbance at 492 nm.
When o-phenylenediamine is employed as a color developer, the absorbance at 492 nm is measured.
The solution was added dropwise into a solution of 17mg of o-phenylenediamine in dichloromethane under ice-cooling.
Subsequently, the peroxidase substrate o-phenylenediamine dihydrochloride was added and the absorbance measured at 450 nm.
The antibody-antigen reaction was observed adding to each well 50 µl of O-phenylenediamine dihydrochloride.
The absorbance of the oxidized o-phenylenediamine substrate at 492 nm was recorded.
After washing as described above, the reaction was developed with O-phenylenediamine ( Zymed ).
Benzimidazole is produced by condensation of o-phenylenediamine with formic acid, [ 2 ] or the equivalent trimethyl orthoformate,.
The peroxidase is then determined by a suitable substrate, e.g., with o-phenylenediamine.
Mg/kg or less of o-phenylenediamine and.
The method according to claim 12, wherein the diamine derivative is o-phenylenediamine.
Conversion of peroxidase substrate O-phenylenediamine is masured at 492 nm.
Ammonia may be added at from 3 to 15 molar equivalents relative to the starting o-phenylenediamine ( OPD ).
Conversion of peroxidase substrate O-phenylenediamine is measured at 492 nm.
Bound antibodies were detected by horse radish peroxidase-protein A ( Amersham ) followed by o-phenylenediamine and measuring A492.
The plates were washed with PBST, and o-phenylenediamine dihydrochloride substrate was added at 100 µl / well.
Color was developed with HRP chromogenic substrate o-phenylenediamine dihydrochloride ( OPD ).
The plates were developed with the substrate O-phenylenediamine dihydrochloride ( Sigma ).
Concentration of free cis-platinum was determined with o-phenylenediamine colorimetric method at 720 nm.
Then 0.2 ml of substrate buffer containing O-phenylenediamine substrate is added.
A range of from 2 to 3 kg / t for a surfactant such as o-phenylenediamine ( OPD ) is preferred.
The colored reaction uses o-phenylenediamine ( OPD ).