Examples of 'oligo dt' in a sentence
Meaning of "oligo dt"
oligo dt: Oligo dt typically refers to a short, single-stranded DNA molecule that is synthesized in a laboratory setting. In the context of molecular biology, oligo dt is commonly used as a primer in reverse transcription reactions to initiate the synthesis of complementary DNA (cDNA) from an RNA template
How to use "oligo dt" in a sentence
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oligo dt
The oligo dT primer was removed by selective precipitation.
In some embodiments the primers are random primers or oligo dT primers.
No oligo dT purification was performed.
Reverse transcription was performed using both oligo dT and random hexamer primers.
Other types of primers with different composition can be used in place of oligo dT.
Both oligo dT and random hexamers were used to generate the first strand product.
One particular example of such a coated solid support includes an oligo dT column.
The total RNA was applied to an oligo dT cellulose column to purify mRNA.
SscDNA was synthesized by priming reverse transcription with oligo dT.
RNA is isolated by chromatography on an oligo dT column and then eluted therefrom.
Total RNA was prepared from excised tissues and then reverse transcribed using oligo dT priming.
RNA is converted to cDNA using an oligo dT primer and reverse transcriptase.
Total RNA was extracted and reverse transcribed using oligo dT primers.
CDNA is then prepared by using an oligo dT primer and MuLV reverse transcriptase.
First strand cDNA is synthesized from the total RNA using oligo dT as the primer.
See also
Ng cDNA of oligo dT primed total RNA was added to each real time PCR.
First strand cDNA was synthesized from the total RNA using oligo dT as the primer.
The RNA was used to synthesize oligo dT primed cDNA according to the manufacturer 's directions.
MRNA was isolated from total RNA from each organism using an oligo dT cellulose resin.
The anchored oligo dT was used as the initial 3 ' primer for RNA transcription to cDNA.
CDNA synthesis was carried out with oligo dT primers and Taq polymerase.
By binding to a solid support, consisting of oligo dT.
CDNA synthesis was primed using oligo dT and / or random hexamers.
First-strand cDNA were synthesized by reverse transcriptase and oligo dT.
Messenger RNA was prepared from the total RNA using oligo dT cellulose affinity purification.
The method according to claim 7, wherein said reagent solution further comprises oligo dT.
RT Mix 2 contained oligo dT as the primers.
Poly A RNA was isolated from total RNA by chromatography on oligo dT cellulose.
An oligo dT primed cDNA library was constructed in λgt10 using techniques described in example 7.
Poly A RNA was isolated from total RNA by chromatrography on oligo dT cellulose.
The length of oligo dT can vary from 8 bases to 30 bases.
RNAse H digest of the RNA strand and elution from the oligo dT cellulose.
Poly rA and oligo dT were prepared as a stock solution which was kept at -20°C.
From this, mRNA was isolated by binding to an oligo dT column.
Next, poly A ⁺ RNA was isolated by oligo dT cellulose chromatography ( literature 3 ).
In some aspects, the target-specific sequence of the oligonucleotide tag comprises an oligo dT sequence.
Oligo dT primer ( product name ) ( manufactured by Takara Bio Inc. ) was used as a primer.
Essentially, double-stranded cDNA was synthesized by oligo dT and random priming.
Poly A+ or mRNA was purified by oligo dT cellulose chromatography ( Strategene, San Diego, CA ).
RNA is isolated from the selected B-cells and converted into cDNA using oligo dT.
Briefly, the first cDNA was constructed using an oligo dT primer with Xho I adapters.
Complementary DNA ( cDNA ) was synthesized from total RNA using reverse transcriptase primed with oligo dT.
Poly A+RNA was selected using oligo dT cellulose.
The mixture according to claim 1, further comprising at least oligo dT.
Samples were incubated 10 minutes at 42 C to allow the oligo dT to anneal to the mRNA.
All libraries were 3 ' generated from mRNA purified on oligo dT columns.
Poly A ⁺ RNA was prepared by binding total RNA to oligo dT cellulose.
The RNA was prepared from MCF-7 cells, then reverse transcribed using oligo dT primers.
The label-tag region being 5 ' of the oligo dT region.
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