Examples of 'oligonucleotide primer' in a sentence
Meaning of "oligonucleotide primer"
An oligonucleotide primer is a short, single-stranded DNA molecule that serves as a starting point for DNA synthesis
How to use "oligonucleotide primer" in a sentence
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oligonucleotide primer
An oligonucleotide primer for amplification of a target nucleic acid.
The reagents comprise an oligonucleotide primer and a polymerase.
Oligonucleotide primer binding sites are underlined and labeled.
The label may be part of the oligonucleotide primer.
The oligonucleotide primer can be either natural or synthetic.
Also disclosed are kits containing a modified oligonucleotide primer.
The first oligonucleotide primer can be a universal oligonucleotide primer.
Elongation of the oligonucleotide primer.
The oligonucleotide primer is labeled with a fluorescently detectable marker.
It is convenient to determine the presence of an oligonucleotide primer extended product.
An oligonucleotide primer bearing the desired mutated sequence is synthetically prepared.
Also disclosed are detectably labeled oligonucleotide primer and probe molecules.
Each oligonucleotide primer within each set differs by one base pair.
Therefore location of modification depends on the sequence of the oligonucleotide primer.
Means to synthesize oligonucleotide primer pairs are well known in the art.
See also
The modified phosphoramidite can be added to any position of the oligonucleotide primer.
Synthetic DNAs can be made by oligonucleotide primer extension across this region.
The target nucleic acid serves as a template for the oligonucleotide primer.
Below each are the oligonucleotide primer positions used in constructing the cDNAs.
Both sequences are flanked by the same sequence for oligonucleotide primer binding.
The modified oligonucleotide primer has at least one modified internucleotide linkage.
Also described herein are detectably labeled oligonucleotide primer and probe molecules.
An oligonucleotide primer sequence need not reflect the exact sequence of the template.
The improvement comprises using a modified oligonucleotide in place of the first oligonucleotide primer.
Both a forward and a reverse oligonucleotide primer were designed in accordance with the present methods.
In some embodiments the amplifying step is performed in the presence of a second oligonucleotide primer.
Hybridization of the oligonucleotide primer to a nucleic acid sample under stringent conditions is defined below.
The invention also provides detectably labeled oligonucleotide primer and probe molecules.
Exemplary kits comprise oligonucleotide primer pairs each one of which specifically hybridizes to a distinct polynucleotide.
In certain cases solid phase amplification comprises a plurality of different immobilized oligonucleotide primer species.
Also disclosed is an oligonucleotide primer for amplifying an Flaviviridae nucleic acid sequence.
Controlled degradation of the modified oligonucleotide provides for production of oligonucleotide primer that is extendable.
The oligonucleotide primer sequences were based on the published sequence in the Gene Bank.
A unique SalI site was inserted into the BamHI site using a synthetic oligonucleotide primer.
Oligonucleotide primer sequences and the conditions for the PCR were in accordance with the literature.
The nucleic acid sequence is substituting by synthesizing an oligonucleotide primer that contains the mutation.
Designed oligonucleotide primer for PCR.
More preferably the randomly primed amplification includes the use of a degenerate oligonucleotide primer.
DNA was genotyped using one fluorescently labeled oligonucleotide primer and one unlabeled oligonucleotide primer.
This preferably takes place before additional nucleotide analogs are incorporated into the oligonucleotide primer.
A target DNA is denatured and an oligonucleotide primer is annealed to the target DNA.
An oligonucleotide primer site in reverse orientation, and.
The modification permits the use of a third oligonucleotide primer in a PCR reaction.
An oligonucleotide primer selected from the group consisting of,.
A template DNA is denatured and an oligonucleotide primer is annealed to the template DNA.
Alternatively, or in addition, the detectable element is linked to the oligonucleotide primer.
Annealing a specific oligonucleotide primer to each specific known sequence ;.
More preferably, the amplifying includes the use of a degenerate oligonucleotide primer.
Thus, binding of the oligonucleotide primer to irrelevant polynucleotides is minimized.
A polynucleotide according to claim 19 which is an oligonucleotide primer for elongation.
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Examples of using Primer
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The primer includes the following modules
It was mostly gray primer and fender fill
A primer is not necessary for small repairs
