Examples of 'oligonucleotide tag' in a sentence
Meaning of "oligonucleotide tag"
oligonucleotide tag: A short sequence of nucleotides that is used to label or mark DNA or RNA fragments. Oligonucleotide tags are commonly used in molecular biology for various purposes such as sequencing, detection, or identification of genetic material
How to use "oligonucleotide tag" in a sentence
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oligonucleotide tag
The oligonucleotide tag may be single stranded.
Single compounds may individually be attached to a unique oligonucleotide tag.
At least one oligonucleotide tag is added enzymatically.
In some embodiments the primer binds to the oligonucleotide tag.
The oligonucleotide tag may be double stranded.
Each antibody is coupled to a unique oligonucleotide tag of known sequence.
The oligonucleotide tag may comprise an adapter region.
The unique identifier region can be in the overhang portion of the hairpin oligonucleotide tag.
The oligonucleotide tag may comprise a secondary structure.
The target specific region can enable attachment of the oligonucleotide tag to the molecule.
The oligonucleotide tag can be linear.
The identifier oligonucleotide can optionally further comprise flanking regions around the oligonucleotide tag.
The oligonucleotide tag can comprise a target specific region.
These vectors generally have two such oligonucleotide tag sequences flanking each cloning site.
The oligonucleotide tag can be attached to any region of a molecule.
See also
One can also amplify the oligonucleotide tag by PCR.
The hairpin oligonucleotide tag may comprise an oligodT sequence.
The PCR primer binding site is preferably designed into the encoding oligonucleotide tag.
Attachment of the oligonucleotide tag to a molecule can occur by ligation.
The PCR primer binding site is preferably designed into the identifier oligonucleotide tag.
In some embodiments the oligonucleotide tag further comprises a label.
The oligonucleotide tag had an EcoRI site therein to facilitate later cloning procedures.
The loop portion of the hairpin oligonucleotide tag may comprise a unique identifier region.
The oligonucleotide tag may further comprise a restriction enzyme recognition site.
In some instances, the hairpin oligonucleotide tag does not comprise a label.
The oligonucleotide tag can be at least one nucleotide in length.
In some instances, both ends of the oligonucleotide tag are attached to the molecule.
The oligonucleotide tag may comprise one or more labels.
Consequently, only the EcoRI site in the oligonucleotide tag was susceptible to EcoRI digestion.
Another oligonucleotide tag can be used for another mixture of compounds.
In some aspects, the target-specific sequence of the oligonucleotide tag comprises an oligo dT sequence.
The oligonucleotide tag may comprise a ribonucleic acid that is cytosine.
The target-specific sequence of the oligonucleotide tag may be specific for a plurality of targets.
The oligonucleotide tag may comprise a ribonucleic acid that is uracil.
Accordingly, a first reactive compound building block or a first identifier oligonucleotide tag can be protected.
In some embodiments an oligonucleotide tag can comprise a cutback in a target molecule.
One or both of the strands of the double-stranded molecule can comprise the oligonucleotide tag.
The oligonucleotide tag can comprise one or more nucleotide moieties.
In one embodiment, the PCR primer sequence is designed into the encoding oligonucleotide tag.
The hairpin oligonucleotide tag may comprise a unique identifier region.
Producing a labeled-RNA molecule can comprise attaching the oligonucleotide tag to the RNA molecule.
In some embodiments the oligonucleotide tag is at least one nucleotide in length.
FIG 4 the first building block is a spacer between the solid-support and the oligonucleotide tag.
The oligonucleotide tag may be comprise one or more secondary structures.
In some instances, the oligonucleotide tag is single-stranded.
The oligonucleotide tag may comprise a ribonucleic acid that is guanosine.
In other instances, the oligonucleotide tag is double-stranded.
The oligonucleotide tag may comprise a ribonucleic acid that is adenine.
The method of claim 1, wherein the oligonucleotide tag is at least 20 nucleotides in length.
An oligonucleotide tag comprising a hairpin may act as a probe for a hybrization chain reaction.
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