Examples of 'overlap extension' in a sentence
Meaning of "overlap extension"
overlap extension: A technique used in molecular biology to splice together DNA fragments by overlapping the ends of the fragments and allowing them to bond, creating a longer continuous strand
How to use "overlap extension" in a sentence
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overlap extension
A first adaptor carrying an overlap extension and barcode sequence is added to a first cDNA.
The insertion of the restriction sites was done by overlap extension PCR.
Overlap extension PCR requires the use of two pairs of primers.
They were assembled from oligonucleotides using a PCR overlap extension method.
This is called overlap extension PCR amplification.
These two PCR products contained common sequence in terminal for overlap extension.
In some embodiments, an overlap extension tail comprises a barcode sequence.
The barcode is then physically linked to the target molecule by overlap extension PCR.
The strand overlap extension products were used for cdh1 deletion.
Point mutations can be introduced into the chimeric product by means of overlap extension PCR.
The product of the overlap extension PCR was amplified using primers pUC-forward and pUC-reverse.
The barcode is then linked to the target nucleic acid sequence using overlap extension PCR.
Mutagenesis was carried out using overlap extension PCR with synthetic primers ( Table 2 ) using standard techniques.
All PCR products were purified by agarose gel electrophoresis prior to overlap extension PCR.
In separate strand overlap extension reactions Horton et al ., Meth.
See also
Figure 5 is a diagram depicting Fv construction by overlap extension.
Using overlap extension PCR, the Trp promoter fragment and the 5 ' end 110 bp fragments were fused.
Briefly, mutations are introduced to insulin coding vectors using overlap extension PCR.
Then, double-stranded DNA was obtained by the overlap extension method using the region.
For each mutation, the two initial PCR products were joined together by overlap extension PCR.
The first VH-VL combination was made by a splice overlap extension PCR reaction.
Mutations were introduced into CAV VP2 sequence by overlap extension PCR.
Construction of site specific mutation N132D was done by the overlap extension method.
Regions deltal-9 were deleted by overlap extension PCR.
Regions delta1-9 were deleted by overlap extension PCR.
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Examples of using Overlap
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Overlap of management areas for each CoC
Some questions may overlap or be too similar
Overlap passes slightly to cover all areas
Examples of using Extension
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This second extension must be authorized by a judge
The applicant shall be informed of any extension
Do not use extension cords or multiple sockets
