Examples of 'passaging' in a sentence
Meaning of "passaging"
Passaging refers to the process of transferring cells or organisms from one vessel to another, typically in a laboratory setting to maintain or grow the culture
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- present participle of passage
How to use "passaging" in a sentence
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passaging
You need extended passaging with antitryptic activity.
Passaging may also be effected to increase cell number.
This action is called subculturing or passaging the cells.
Continued passaging in this manner can continue indefinitely.
The distribution of chromosomes did not change with passaging.
The passaging was repeated once more.
The cells are expanded in number by passaging.
Three passaging strategies were used.
Expanding by consecutively passaging the cells.
Passaging of the virus to attenuation was accomplished using classical virology methods.
The cells are cultured to near confluence before additional passaging.
Repeated serial passaging results in the eventual recovery of avirulent bacteria.
For therapy experiments tumours are maintained vÃa passaging of tumour fragments.
The further passaging is especially neutral or relaxed passaging as previously described.
Chondrocyte phenotypic stability is gradually lost in mature chondrocytes upon passaging.
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Passaging is typically done when the cells reach a certain density in culture.
The high yield phenotype of the reassortant is selected by multiple passaging.
Cells were grown through an initial passaging and then cloned by limiting dilution.
Passaging of equine influenza virus preferably takes place in embryonated chicken eggs.
The method further includes passaging the cultured cells prior to confluence.
The passaging may be carried out in other cell or organ culture systems.
One of these samples was used for immunostaining four days after passaging.
Passaging may be conducted under specific environmental conditions to select for attenuated cells.
Supernatants from five rounds of virus passaging were subjected to standard virus plaque assays.
Passaging and harvesting formulation and method for human pluripotent stem cells.
The cells maintain a consistent normal karyotype notwithstanding repeated passaging in certain embodiments.
Passaging in the presence of said polyclonal antibodies was repeated for two more times.
Examples hereof are chondrocytes that have undergone extensive passaging and stem cells.
Passaging at daily intervals ensured that the virus was always in a logarithmic phase of replication.
Prolonged compound treatment and cell passaging dramatically increased the number of reprogrammed colonies.
These results indicate that the deletions were maintained stably during in vitro passaging on PAMs.
Any residual cells from the passaging procedure also were cryopreserved for future use.
Transfer into a new flask with fresh growth media for passaging the cells.
Such passaging techniques can be performed with any known tumor or cancer cell lines.
Still further enrichment is realized upon passaging the cultures of the previously frozen cells.
Amongst the common manipulations carried out on culture cells are media changes and passaging cells.
Methods for attenuating include passaging a virulent strain multiple times or exposure to irradiation or chemicals.
The attenuation of the CSFV can be done by serial passaging.
Passaging on Vero cells gives a high virus yield.
Bacterial culture and in vivo passaging of Listeria coli were cultured following standard methods.
Passaging in static cultures is maintained until a NHA cultivar is produced.
DNA and RNA were extracted from a fraction of the cells upon passaging.
An example of a passaging procedure is disclosed in detail in the Examples section.
Cryopreservation and / or proliferation or passaging before transplantation.
Passaging on Vero cell cultures may be used to attenuate the virus.
Schematic of the RNP-transfection and passaging experiments.
Passaging of cells continued until they showed typical human ES morphology.
In some embodiments, the propagating comprises passaging the cell.
Passaging a rotavirus preparation on a suitable cell line ;.
An isolated cell according to claim 1 or 2 which maintains a normal karyotype with passaging.
