Examples of 'pbluescript' in a sentence
Meaning of "pbluescript"
pbluescript (adjective) - This phrase may refer to a specific type of molecular biology plasmid vector named pBluescript, typically used in genetic engineering and cloning experiments
How to use "pbluescript" in a sentence
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pbluescript
Preparation of the pBluescript vector part.
The ori sequence inserted comes from pBluescript.
The template used was pBluescript plasmid containing the cloned gene from the cDNA library.
A control ligation contained only the linearized pBluescript.
PBluescript vectors containing cDNA clones were obtained.
The resulting fragments were randomly cloned into pBluescript and sequenced.
The cloning vector pBluescript was obtained from Stratagene.
The inserts were subcloned into the plasmid vector pBluescript and sequenced.
The cloning vectors pBluescript and pBluescribe were obtained from Stratagene.
Mass excisions can be performed on these libraries to generate libraries in the pBluescript phagemid.
These cDNA were excised on plasmid pBluescript SK using the method recommended by Stratagene.
Positive phage are isolated and purified and the insert is subcloned into pBluescript.
The PCR product was cloned into pBluescript using ClaI linkers.
Representative cDNA inserts for both size classes were subcloned into the plasmid vector pBluescript SK.
PCR fragments were cloned into pBluescript and JE sequences were verified by nucleotide sequencing.
See also
The XbaI site was created only for cloning into the polylinker of the pBluescript vector.
Amplified fragments were subcloned in pBluescript and 4 independent clones were sequenced on both strands.
An exemplary plasmid vector which may be used is pBluescript II.
The resulting fragment was subcloned into pBluescript in the KpnI and BamHI sites, creating pBS-Nde.
Each cDNA was subcloned into the EcoRI site of pBluescript SK.
When necessary, pBluescript is used to balance the quantity of DNA.
The PCR products were subcloned into pBluescript for sequencing.
A pBluescript library clone containing no insert was used as a negative control ( NEG ).
The PCR product was subcloned into pBluescript for sequencing.
The amplified DNA fragment was purified, digested with XhoI and NotI, and ligated into pBluescript.
The cstIII gene was then sub-cloned in a pBluescript plasmid upstream cgtAII.
The DNA fragments generated by PCR were gelpurified and subcloned into pBluescript.
The negative control ( NEG lane ) was a pBluescript library clone without an insert.
Positive BAC clones have been found and subcloned into pBluescript KS.
DNA fragments were subcloned into pBluescript KS - and sequenced along both strands by primer walking.
This cDNA was recovered by PCR amplification from the vector pBluescript containing it.
For blunt end-cloning pBluescript digested with Eco RV was used.
These ligated fragments were cloned into the vector pBluescript II KS.
This fragment was subcloned into pBlueScript ( II ) KS and its nucleotide sequence was determined.
The letter represents the promoter of the lac of the vector pBluescript II SK.
Full length NS3 gene was cloned into pBluescript II vector without modifications.
The resultant DNA fragment was cloned into the EcoRV gap of pBluescript II SK.
PBluescript ( Stratagene Cloning Systems ) was used for construction of a cDNA library.
The resulting PCR product was cloned into pBluescript and sequenced.
PBluescript with full length MN cDNA was cleaved by EcoRI and blunt ended by S1 nuclease.
Chromosomal DNA digested by Cloning in pBluescript by.
The cloning vectors pBluescript and PCR script were obtained from Stratagene, USA.
By recombination events, this results in the construction of a pBluescript plasmid.
Using pBluescript as the cloning vector, it was possible to subclone the 25 kb fragment.
Upon conversion, cCNA inserts will be contained in the plasmid vector pBluescript.
The resultant fragment is then ligated into pBluescript II KS+digested with Eco RV.
Upon conversion, cDNA inserts will be contained in the plasmid vector pBluescript.
This insert was cloned into pBluescript and the resulting vector is called pSC60.
For sequencing, each gene fragment was subcloned into the pUC vector or pBluescript vector.
All six pBluescript plasmids obtained in this way contained the same 2.5 kb insert.
