Examples of 'phage library' in a sentence
Meaning of "phage library"
phage library ~ a collection of bacteriophages, viruses that infect and replicate within bacteria
How to use "phage library" in a sentence
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phage library
Amplification and selection of phage library.
An scFv display phage library can be prepared as described below.
Degenerate oligonucleotides for combinatorial phage library generation.
The phage library can be created in a variety of different ways.
The pCANTAB vector was used for phage library construction.
A phage library was screened for the ability to bind to biotinylated BLyS.
Screening of phage library.
A phage library can be prepared by using various immunoglobulin genes as sources.
The immunoglobulin can be isolated from a phage library see McCafferty al.
The phage library mixtures were administered to mice vÃa tail vein injection.
Exemplary monoclonal antibodies derived from a phage library are provided herein.
Aliquots of the phage library are stored for future amplification and use.
Phagemid vectors are often employed for constructing the phage library.
This new phage library will be a valuable source of antibodies to essentially any target.
Collectively these phages were used to prepare an enriched phage library as described earlier.
See also
The phage library selection was intended to select mutants with high affinity for antigen.
Plaque hybridizations to screen the phage library were performed with a stringent wash ofO.
Immunoscreening was performed using the above constructed canine testis cDNA phage library.
Phage library was then made by infection the mutational library with the helper phage.
The human antibody may be prepared from the human antibody phage library.
This step was repeated once and depleted phage library supernatants were reserved for panning.
The disclosed methods may include contacting isolated oocytes with a phage library.
Phage library was incubated with biotinylated target protein at RT.
Monoclonal antibodies can also be produced using well known phage library systems.
Screening of the phage library for TEL binders was performed by panning.
The cells producing the antibodies are a source of genetic material for making a phage library.
The phage library was screened with antibodies from autoimmune patients with Type I diabetes.
Immunoscreening was carried out using the canine testis cDNA phage library prepared above.
Alternatively, a phage library can be employed.
This reflects the very limited size of the light chain repertoire in the phage library.
Figure 3 shows phage library design and sequences of selected clones.
Million clones of a placenta lambda phage library Stratagene.
In this case, the phage library is incubated with the immobilized target.
The immunoglobulin can be isolated from a phage library see McCafferty et a /.
For phage library screening, the same phage library was used as already described.
First, separation of a specific clone from an scFv display phage library is described.
In alternative embodiments, the phage library may be prescreened against a control cell, tissue or organ.
According to another aspect of the invention, they are obtained by the phage library method.
Thus, a phage library may be a population of particles enriched for a preselected ligand binding specificity.
For example, the antibody fragments can be isolated from an antibody phage library.
A phage library was selected using soluble biotinylated antigen and streptavidin-coated paramagnetic beads.
Using various types of immunoglobulin genes as sources, a phage library can be also created.
Using the dog testis-derived cDNA phage library prepared as described above, immunoscreening was carried out.
This in vivo antibody repertoire selection is mirrored in the eventual " naive " phage library.
Transforming breast cancer cells with the phage library in the presence of mitotic inhibitors;.
Furthermore, techniques to obtain human antibodies by panning with a human antibody phage library are known.
For phage library selection, soluble CEA antigen was biotinylated.
The binding characteristics of the members of the antibody phage library can be pre-determined.
The library may be a phage library, for example, a scFv phage library or a Fab phage library.
Furthermore, techniques for obtaining human antibodies by panning with a human antibody phage library are known.
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