Examples of 'phenol-chloroform' in a sentence
Meaning of "phenol-chloroform"
phenol-chloroform (noun) - a mixture of phenol and chloroform commonly used in molecular biology for DNA or RNA extraction, acts as an organic solvent to separate biomolecules
How to use "phenol-chloroform" in a sentence
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phenol-chloroform
Than just a normal phenol-chloroform extraction.
Two phenol-chloroform extractions were performed.
Proteins were removed by phenol-chloroform extraction.
A standard phenol-chloroform purification is applied to the sample.
The incubated mixture was treated by phenol-chloroform extraction.
Phenol-chloroform extraction and ethanol precipitation was carried out.
Lysis was followed by several phenol-chloroform extractions and ethanol precipitations.
Once digested, it was purified by electroelution and then extracted with phenol-chloroform.
It is then purified by phenol-chloroform extraction and ethanol precipitation.
To the liquid inside each dialysis tube was added a phenol-chloroform mixture.
The samples were phenol-chloroform extracted and isopropanol precipitated.
After amplification the reaction solution was extracted twice with phenol-chloroform.
The reaction mixture was subjected to phenol-chloroform extraction followed by ethanol precipitation.
Then, TdT was denatured and inactivated by the addition of a phenol-chloroform mixture.
Samples were extracted with phenol-chloroform and chloroform, followed by ethanol precipitation.
See also
In the samples from the other groups we used the phenol-chloroform standard.
A second phenol-chloroform extraction was carried out, followed by a precipitation with ethanol.
This procedure was repeated twice so that three phenol-chloroform isoamylalcohol extractions were done.
Then phenol-chloroform was added and aqueous phase was washed twice with chloroform.
Virion RNAs were extracted with phenol-chloroform and collected by ethanol precipitation.
The method of claim 2 wherein the step of extracting comprises a phenol-chloroform extraction.
Following phenol-chloroform extraction, DNA was recovered by ethanol precipitation.
Following linearization, the enzyme is inactivated by phenol-chloroform precipitation and removed.
After phenol-chloroform extraction, a DNA fragment was recovered by ethanl precipitation.
This extraction procedure with the phenol-chloroform mixture was repeated twice more.
The supernatant is pipetted into a fresh tube and a further 1 volume of phenol-chloroform was added.
Further, the reaction mixture was subjected to phenol-chloroform extraction followed by ethanol precipitation.
This process was performed applying classical methods of extraction with guanidine thiocyanate phenol-chloroform.
Incubation with a restriction enzyme was always followed by phenol-chloroform extraction and ethanol precipitation.
After phenol-chloroform extraction, a DNA fragment was recovered by ethanol precipitation.
The bacterial genome was purified from each sample by standard phenol-chloroform extraction method.
After extraction with phenol-chloroform the DNA was precipitated by ethanol.
The supernatant was recovered and subsequently extracted with phenol, phenol-chloroform and chloroform.
PCR products were subjected to phenol-chloroform extraction and ethanol precipitation.
Anion-exchange chromatography offers higher purity and more consistent results than phenol-chloroform extraction.
After the lysis, the DNAs are extracted with phenol-chloroform mixture and precipitated with ethanol.
The CIP enzyme is thereafter inactivated by two successive extractions with phenol-chloroform.
Subsequently, this procedure for extraction with the phenol-chloroform mixture was repeated twice more.
Phenol-chloroform extraction and ethanol precipitation were carried out using the reaction solution to recover DNA.
DNA was extracted using the standard phenol-chloroform method.
The DNA was isolated by phenol-chloroform extraction followed by ethanol precipitation.
DNA was purified by CsCL gradient followed by phenol-chloroform extraction.
DNA was extracted by phenol-Chloroform procedure and finally precipitated with ethanol.
DNA can subsequently be purified by the usual phenol-chloroform extractions.
The reaction was terminated by phenol-chloroform extraction, the DNA fragment was recovered by ethanol precipitation.
The band of DNA was extracted from the gel phenol-chloroform extractions.
Subsequent treatment with a phenol-chloroform mixture and precipitation with ethanol gave linear DNA.
DNA from the positive clones was purified by CsCL and followed by phenol-chloroform extraction.
PCR products were purified by phenol-chloroform extraction and ethanol precipitation.
The total intracellular RNAs were then isolated by guanidinium isothiocyanate and phenol-chloroform extraction ( Stratagene ).
