Examples of 'phosphotyrosine' in a sentence
Meaning of "phosphotyrosine"
Phosphotyrosine is a modified form of the amino acid tyrosine, typically found in proteins, where a phosphate group is added to a tyrosine residue. It plays a crucial role in various cellular processes and signaling pathways
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- A phosphorylated derivative of tyrosine that is active in signal transduction and regulation of enzymatic activity
How to use "phosphotyrosine" in a sentence
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phosphotyrosine
Phosphotyrosine can be detected through specific antibodies.
Immunoprecipitates were immunoblotted with antibodies to phosphotyrosine.
The amount of phosphotyrosine present is detected with a densitometer.
The membrane was then incubated with a commercially available antibody directed against phosphotyrosine.
The asterisk indicates the novel phosphotyrosine residue identified.
Antibodies to phosphotyrosine can be produced due to the size of the antigen.
These data indicate successful incorporation of phosphotyrosine into the synthetic peptide moiety.
Examples of phosphotyrosine phosphatase inhibitors include sodium vanadate and the like.
In its phosphorylated form, tyrosine is called phosphotyrosine.
The domains bind to phosphotyrosine containing proteins or peptides with imperfect specificity.
Phosphoaminoacid analysis of p38 demonstrated the presence of a phosphothreonine and phosphotyrosine.
The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins.
Phosphotyrosine and protein levels were determined by Western blotting.
Immunoprecipitation indicated that the phosphotyrosine bands corresponded with the FAK bands on the blots.
Often, the physical association of such regulatory proteins is mediated through those phosphotyrosine residues.
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Those based upon phosphotyrosine have been demonstrated to be the more specific tyrosine kinase inhibitors.
Cell extracts were made, and the reduction in phosphotyrosine was determined by western blot.
Ratios of phosphotyrosine to EGFR protein for wt and mutant receptors.
Phosphotyrosyl proteins were isolated from cell lysates by immunoprecipitation with antibody to phosphotyrosine ( anti-pTyr ).
Direct incorporation of phosphotyrosine without phosphate protection can also be used in Fmoc chemistry.
These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine / threonine and phosphotyrosine residues.
Therefore, the phosphotyrosine mimic-containing compounds are competitive inhibitors of that binding.
Dimerization of WT and mutant EGFRs, and specific phosphotyrosine content of receptor complexes.
Phosphotyrosine phosphatase ( PTP ) plays a role in the regulation of these routes.
Accordingly, the need exists for effective phosphotyrosine mimics as a replacement for the critical phosphotyrosine residue.
Phosphotyrosine and protein levels were determined by western blotting . MTT Proliferation Assay.
This tyrosine kinase activity increases the phosphotyrosine content of many receptor-like and cytoplasmic regulatory proteins.
Phosphotyrosine is estimated by measurement of absorbance at 450 nm.
PTP1B can dephosphorylate the phosphotyrosine residues of the activated insulin receptor kinase.
Fig . 17 illustrates an assay to detect peptides containing phosphotyrosine residues.
The specific stat is bound to phosphotyrosine grouping of receptor-Jak complex, being itself phosphorylated.
The enzyme first wraps around DNA and creates a single, 3 ' phosphotyrosine intermediate.
Proteins containing phosphotyrosine were immunoprecipitated and separated by SDS-PAGE.
Rabbit polyclonal antiserum specific for phosphotyrosine ( anti-Ptyr antibody ).
Moreover, several phosphotyrosine containing proteins were found in Shc immunoprecipitates from PYK2 overexpressing cells.
The SH2 domains enable SHP2 to bind specific phosphotyrosine residues within protein sequences.
The anti-phosphotyrosine antibodies were preincubated with 10mm phosphotyrosine for blocking.
In non-clinical models, inhibition of EGFR phosphotyrosine results in cell stasis and / or death.
Reduction of phosphotyrosine content of EGFR monomers in vivo by T691stop neu coexpression.
Sodium vanadate ( 100 uM ) was then added to inhibit the activity of phosphotyrosine phosphatases.
Immunoblotting analysis with phosphotyrosine anti-bodies following immunoprecipitation of PYK2, PKN and Fak by specific antibodies.
The dotted circles indicate the migration of unlabeled phosphoserine ( pS ), phosphothreonine ( pT ), and phosphotyrosine ( pY ).
The ligand studied was a phosphotyrosine peptide with the sequence NH2-pYEEI-COOH.
Phosphotyrosine containing proteins were visualized by immunoblotting with anti-phosphotyrosine antibody ( Upstate Biotechnology, Inc. ).
In a preferred embodiment, the anti-IGF-IR antibody causes a reduction in phosphotyrosine signal by at least 20 %.
Phosphotyrosine in measured by absorbance at 450 nm.
However, at pH 9.4, alkaline phosphatase 1 is more effective than ALPase 2 and exhibits a preference for phosphotyrosine.
The cell lysate were used for phosphotyrosine AXL enzyme-linked immunosorbant assay ( pY-AXL-ELISA ).
Immuno detection of EGFR protein ( top ) and phosphotyrosine ( bottom ).
Fig . 7a shows the results of a HER-3 phosphotyrosine ELISA performed with anti-HER-3 antibodies of the invention.
