Examples of 'plasmid construction' in a sentence
Meaning of "plasmid construction"
plasmid construction: Plasmid construction involves the process of creating recombinant DNA molecules by inserting specific genes or DNA sequences into plasmids. This phrase is commonly used in molecular biology and genetic engineering experiments
How to use "plasmid construction" in a sentence
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plasmid construction
The resulting plasmid construction is amplified in bacteria.
Standard methods were used for plasmid construction.
The effector plasmid construction is described above.
The underlined BglII site is added for plasmid construction purposes.
Plasmid construction and disruption of the acetyltransferase gene.
This clone had integrated a single copy of the viral plasmid construction.
The plasmid construction strategy is as follows.
Boxes indicate the restriction sites used for the plasmid construction.
Plasmid construction for epitope mapping.
Both direct cloning and PCR were used for plasmid construction.
Each plasmid construction was assayed in from two to five separate electroporation experiments.
Selection of the target sequence and plasmid construction were based on manufacturer 's recommendations.
Plasmid construction and stable ES cells.
A schematic diagram of plasmid construction is shown in Figure 4.
Plasmid Construction Three types of IF protein constructs were made and analyzed in this report.
See also
The schematic representation of this new plasmid construction is also shown in Figure 5.
The plasmid construction strategy is described as follows,.
Figure 5 is an illustration of a recombinant plasmid construction of VSV-G protein without signal sequence.
The plasmid construction strategy is described as follow,.
Thus, pVY1 was cleaved with Bgl II and submitted to the following recombinant plasmid construction.
Therefore, plasmid construction allowing high level expression of the recombinant antigen was made as follows.
In the producing cell line, the Cre protein is expressed from the proviral plasmid construction.
An example of plasmid construction allowing said expression is described in FIG . 1.
PVY1 was cleaved with Bgl II and submitted to the following recombinant plasmid construction.
Plasmid construction of the Tau-fused bait.
Standard methods were used for plasmid construction ( Ausubel et al . ).
Plasmid construction and transformation of Agrobacterium are described in Example 7.
Figure 12, Final bi-cistronic plasmid construction used for the stable transfection of CHO cells.
Plasmid construction All expression plasmids are of the C-POT type.
One of these plasmids, designated pAc 373 SSBXP-8 was utilized for the final plasmid construction.
Plasmid construction and immunoadaptertoxin expression All primers and oligonucleotides were purchased from Metabion ( Martinsried, Germany ).
Example 1, Plasmid construction.
Plasmid Construction Construction of plasmids containing LF-PE fusions was performed as follows.
Fig . 1 shows a plasmid construction scheme for the plasmid pMDSG.
Plasmid construction and nucleic acid sequences are shown in FIGS . 2 and 3.
In particular, this figure shows a plasmid construction to generate a S . typhimurium-derived minicell producing strain.
Each plasmid construction was assayed 2-5 times in separate electroporation experiments.
Fig . 2 shows a plasmid construction scheme for the plasmids pMMGR and pMMifa.
Plasmid Construction A cassette approach is used to independently construct portions of the APP-REP plasmid ( Figure 2 ).
Figure 5 depicts the plasmid construction of the expression vectors for Humanized 1308.
EXAMPLE 2 Plasmid construction DNA manipulation techniques used were as described by Sambrook et al.
Appropriate plasmid construction was confirmed by digestion with EcoRI and 0.8 % agarose gel electrophoresis.
Example 2 Plasmid Construction A schematic representation of the expression plasmid is shown in Fig . 1.
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