Examples of 'plasmid was digested' in a sentence
Meaning of "plasmid was digested"
Plasmid was digested: Describes a scientific procedure where a plasmid DNA molecule is cleaved at specific sequences by restriction enzymes
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plasmid was digested
The plasmid was digested by restriction enzyme EcoRI.
PEm was digested with BamHI and the linearized plasmid was digested with calf intestinal phosphatase.
This plasmid was digested with Bam HI.
Likewise, the pNATABH,, Herceptin HC plasmid was digested with SacII and XhoI.
This plasmid was digested with the restriction enzymes sma I and EcoR I.
For expression in insect cells, the pBud-SLURP-1 plasmid was digested by Hind III and EcoR V.
The resulting plasmid was digested with restriction enzymes EcoRI and SalI.
The polymerase was heat-inactivated for 10 minutes at 75°C and the plasmid was digested with Nhe I.
The resulting plasmid was digested with AgeI.
This plasmid was digested with Bam HI to release the a-galactosidase cDNA.
The purified plasmid was digested with HindIII.
The plasmid was digested with Stul and Not I, followed by blunting and re-ligation.
The resulting plasmid was digested with NheI and XmaI.
This plasmid was digested with SalI, and the fragments were separated on a 1 % agarose gel.
The resulting plasmid was digested with Kpnl and Xbal.
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Each plasmid was digested by BsiWI restriction enzyme to extract insert VI ( 400 bp ).
The resulting plasmid was digested with Nhel and Xmal.
Each plasmid was digested by EcoRV restriction enzyme to extract insert VH ( 400 bp ).
The speA carried on a E . coli plasmid was digested with restriction enzymes HindIII and SalI.
This new plasmid was digested with BglII and SalI to recover a fragment of 3.0 kb.
Typically 40 µg of circular plasmid was digested in a volume of 400 µl restriction buffer.
Next, this plasmid was digested with Hind III and ClaI to prepare a 761 bp fragment.
Thirty µg of purified plasmid was digested with SpeI for 1 hr at 37°C.
The obtained plasmid was digested with Sal I to obtain a DNA fragment containing ddh.
After assembling, the plasmid was digested with the unique restriction enzyme BamHI.
Further, this plasmid was digested with XhoI and XbaI to obtain an XhoI-XbaI 1.7 kb fragment.
Then, this plasmid was digested with SalI.
The thus obtained plasmid was digested with restriction enzymes XbaI and SalI.
The obtained plasmid was digested with restriction enzymes Hid III and Eco RI.
Then, this plasmid was digested with Sall.
The non-mutated parental plasmid was digested using DpnI, which only cuts methylated DNA.
Then, the plasmid was digested with Bam HI.
After that, the plasmid was digested at a Sac I site.
The pUC18 plasmid was digested in the same way.
The pACYC184 plasmid was digested with restriction enzymes XbaI and HindIII.
The G5BCAT plasmid was digested with the EcoR1 restriction enzyme.
The pSV2-dhfr plasmid was digested with restriction enzyme BglII ( 3 ' end of the dhfr gene ).
The linearized plasmid was digested with BAL 31 ;.
The DNA ( 5 µl ) from each plasmid was digested with EcoRI in 20 µl total volume.
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Examples of using Digested
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You just digested the bad guy
Maybe that piece of paper was not digested
He digested more of his food before he threw up
Examples of using Plasmid
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Said plasmid contains a tetracycline resistance gene
The construction of this plasmid is described below
Deposit of plasmid containing carcinogenic substance adducts
