Examples of 'poly-a' in a sentence
Meaning of "poly-a"
poly-a (noun) - a molecular biology term referring to a nucleic acid sequence composed of multiple adenine bases
How to use "poly-a" in a sentence
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poly-a
Polyadenylation is carried out by a poly-A polymerase.
The length of the poly-A tail is not particularly limited.
There is also a polyadenylation signal sequence and a poly-A tail.
The poly-A tail is thought to have a function in stabilizing the transcript.
Lysines at the carboxy terminus correspond to the poly-A region.
The poly-A tail is said to have a function in stabilizing the transcripts.
Expression vectors normally comprise at least a promoter and a poly-A signal.
It is thought that the poly-A tail has a function in stabilizing the transcripts.
This finding has important implications for the use of poly-A regions.
The poly-A tail is essential for keeping the mRNA from being degraded.
And downstream of the encoding sequence a poly-A sequence followed by a transcription termination site.
This digestion removes the minimal promoter but retains the poly-A end.
A cDNA library is prepared from poly-A mRNA extracted from the CTL clones.
The nucleic acid construct of any one of the preceding claims further comprising a poly-A sequence.
A cDNA library is prepared from poly-A mRNA extracted from allo-restricted CTL clones.
See also
Mammalian cells may also require terminator sequences and poly-A addition sequences.
Poly-A mRNA is isolated from BCE cells whose proliferation has previously been inhibited by endostatin.
As mentioned above, the RNA molecule of the present invention may also harbor a poly-A tail.
From the whole RNA separated, poly-A tailed RNA is fractionated by an ordinary method.
The ENE fragment contains a specific U-rich hairpin structure that interacts with the poly-A tail.
Poly-A tailed RNA is fractionated from the RNA thus recovered by a conventional method.
The Selexis vectors included a CMV promoter and a matching poly-A signal.
CDNA is synthesized from the poly-A tailed RNA thus obtained by a conventional method.
Poly-A RNA was isolated from the total RNA by chromatography.
A cDNA library is prepared from poly-A mRNA extracted from the Aspergillus - specific T cell clones.
In some embodiments, a provided mRNA comprises a poly-A tail.
A single C embedded in a poly-A DNA hairpin construct.
A poly-A sequence and downstream thereof a transcription termination site ;.
Total RNA was prepared and Poly-A RNA was selected with oligo DT cellulose.
They will form part of the poly-A tail.
A poly-A tail was also attached to mRNA 4 for purification by oligo-dT.
In certain embodiments, the target polynucleotide lacks a poly-A tail.
In the absence of phosphate group, a poly-A or poly-C linker arm is more effective.
In fact, the clone extended all the way to the poly-A tail.
The primers hybridizing to a poly-A sequence are usually oligo-dT primers or Oligo-dU primers.
A target comprises a poly-A tail.
The poly-A and poly-C fragments are more effective than the poly-T fragments.
In general, such purification takes advantage of the poly-A tails on mRNA.
Additionally, or alternatively, poly-A fragments can be removed by binding to streptavidin coated affinity columns.
As a reverse primer, a primer is used that binds to the poly-A tail of mRNAs.
In an especially preferred embodiment the poly-A sequence has a length of approximately 120 adenosine residues.
The position is at or near where the pre-mRNA will have the poly-A tail attached.
Furthermore, a long poly-A sequence of about 120 nucleotides results in optimal transcript stability and translation efficiency.
The 3 ' untranslated region appears to be complete as it includes a poly-adenylation signal and poly-A tail.
Poly-A mRNA is selected by oligo-dT cellulose chromatography.
Appropriate source tissues are immature seeds, immature embryos or mid-maturation stage cotyledons . Poly-A mRNA.
Poly-Q and poly-A have been found in patient aggregates.
This 3 ' OH end was extended with terminal transferase by adding a poly-A tail.
A poly-A tail is commonly added to the 3 ' end of the RNA by a process called polyadenylation.
Transcription is driven by the EF-1 alpha promoter and terminated by a poly-A signal.
