Examples of 'polyadenylated' in a sentence
Meaning of "polyadenylated"
polyadenylated (verb) - Polyadenylation is the process of adding a poly(A) tail to a messenger RNA molecule
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- simple past tense and past participle of polyadenylate
- Reacted to form a salt or ester of polyadenylic acid
How to use "polyadenylated" in a sentence
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polyadenylated
Neither transcript is polyadenylated and both are transcribed by PolIII.
Polyadenylated messenger RNA is isolated from human cells by known methods.
The molecule can be polyadenylated.
The polyadenylated molecule can be a polyadenylated RNA molecule.
There is evidence for multiple polyadenylated sites for this transcript.
Polyadenylated messenger RNA is isolated from human mononuclear leukocytes by known methods.
This leads to the production of a new polyadenylated mRNA that is nonfunctional.
Polyadenylated RNA can be obtained from a sample by methods known in the art.
This is due to the fact that the transcripts are each polyadenylated at different positions.
Polyadenylated RNA was then recovered from total RNA and used for the synthesis of cDNA.
Labeled targets were made from polyadenylated RNA.
This polyadenylated sequence was present at the 3 ′ ends of the two types of clone isolated.
The RNA molecules can be polyadenylated.
Denatured polyadenylated template fragments are ligated to poly ( dT ) oligonucleotides on the surface of a flow cell.
These results indicate that all five conditions yielded polyadenylated FPET RNA of acceptable size.
See also
Polyadenylated RNA was isolated and used to prepare double-stranded cDNA.
The RNA carrier may be a polyadenylated RNA.
If desired, the immobilized polyadenylated fragmented RNA is enriched prior to conversion to cDNA.
These RNA molecules were readily detected in total RNA or in polyadenylated RNA.
The RNA molecule can be a polyadenylated RNA or phage RNA.
Both are flanked by UTRs, both are capped and polyadenylated.
Purification of Polyadenylated mRNA.
In some examples, substantially all of the first single-stranded mRNAs are polyadenylated.
Many eukaryotic non-coding RNAs are always polyadenylated at the end of transcription.
Therefore, cloned DNA will be transcriptionally regulated by the metallothionein promoter and polyadenylated.
Providing a sample comprising polyadenylated RNA from said subject ;.
Also, the two histone genes produced greatly different levels of properly polyadenylated mRNA.
Contacting a sample comprising polyadenylated RNA with said drug candidate ;.
In some embodiments, the mRNA may be polyadenylated.
In a particular embodiment, the polyadenylated fragmented RNA is hybridized to a solid phase bead format.
Alternatively, the mRNA molecules are not polyadenylated.
The AATAAA box close to the polyadenylated 3 ' end of the cDNA is underlined as well.
In some preferred embodiments, the mRNA is polyadenylated.
The isolation of the polyadenylated RNA may be conducted utilising an oligo-dT spun column.
In some examples, the mRNA is polyadenylated.
In yet another embodiment, the polyadenylated fragmented RNA is immobilized before conversion to a cDNA.
Alternatively, the molecule is not polyadenylated.
However, said polyadenylated RNA may also be bound on solid supports like polystyrene beads.
There is also a poly-adenylation signal sequence and a polyadenylated tail.
In one aspect, polyadenylated RNA from a single cell is analyzed by the methods disclosed herein.
In some instances, the kits and compositions are used for stochastically labeling a polyadenylated molecule.
The labelled PNA was incubated with a mixture of polyadenylated RNA 's according to the following procedure.
In some embodiments, target RNA polynucleotides are polyadenylated.
Polyadenylated mRNA was prepared from a D10 cell line and cDNAs were prepared using standard techniques.
In some embodiments, the RNA polynucleotides are not polyadenylated.
Isolating polyadenylated RNA from the test sample ;.
In some embodiments of these methods, the Agent mRNA is polyadenylated.
And, in reaction D, no background polyadenylated DNA molecules were added to the reaction.
Providing a test sample and a control sample each comprising polyadenylated RNA ;.
After isolating polyadenylated mRNA from total RNA, complementary DNA or cDNA is synthesized therefrom.