Examples of 'polylinker' in a sentence
Meaning of "polylinker"
Polylinker (noun): A short segment of DNA that contains multiple restriction enzyme recognition sites, allowing for the insertion of DNA fragments
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- A short sequence of DNA containing several restriction enzyme recognition sites that is contained in cloning vectors
How to use "polylinker" in a sentence
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polylinker
The orientation of the polylinker was verified by sequencing.
It illustrates a universal plasmid containing a polylinker site.
Fragment spanning the polylinker is amplified as follows.
A polylinker was added at the NotI site.
This insert is also surrounded by polylinker sites.
The entire polylinker is flanked by NotI sites.
The neo gene was cloned into the polylinker.
The polylinker site has been indicated.
The amino acids encoded by the polylinker are underlined.
A further useful element in an expression vector is a multiple cloning site or polylinker.
The sequence of the polylinker used is given below.
An intermediate plasmid was constructed in order to remove a redundant polylinker region.
Thisvector is a plasmid comprising a polylinker containing unique restriction sites.
The fragment was inserted in the orientation shown relative to the polylinker.
Other formulations of a plasmid with polylinker sites may be constructed as required.
See also
The HindIII site shown in brackets is destroyed by the polylinker cloning.
This was ligated into the polylinker of pcDB at the EcoRI site.
The successful manipulation was confirmed by sequencing the new polylinker fragment.
The region was replaced by a polylinker to allow for additional cloning sites.
The polylinker may contain more than one type of reactive group.
The nanoparticles may be contacted with a polylinker solution in many ways.
The polylinker sequence is used to introduce an expression cassette for a gene of choice.
Both of these sites were synthesized into the newly added polylinker region.
It contains a polylinker to assist cloning and a region of the octipine synthase terminator.
The SnaBI and NotI sites are located in the polylinker region of the plasmid.
These polylinker sequences are positioned at the second junction of recombinant fragment and expression vector.
The vectors differ as to the position where the polylinker cassette has been inserted.
A portion of the vector polylinker was retained between the two fragments for subsequent additions.
A nanoparticle film may be formed on a substrate and the polylinker solution sprayed on the film.
Several restriction sites for polylinker enzymes within the vector sequences were eliminated by mutageneses.
The XbaI site was created only for cloning into the polylinker of the pBluescript vector.
The polylinker interposed into the expression cassette may be chosen with this site in mind.
Upstream of the promoter was inserted a polylinker region containing sites for cloning candidate fragments.
The polylinker was integrated vía the Sall cleavage site of the vector.
The fragments were cloned into the polylinker region situated between the sequence priming sites.
DNA coding for ten asparagine residues separates the malE gene and the above polylinker.
A STOP codon may be inserted after the polylinker for translation termination.
The polylinker does not interrupt the lac Z reading frame.
The vectors each have a malE linked vía a polylinker to a lac Z gene.
Vectors also typically include a polylinker region containing several restriction sites for insertion of foreign DNA.
This fragment contains the human gamma I gene and flanking polylinker sequences.
The polylinker sequences containing multiple restriction sites are inserted into the lac Z sequence.
One plasmid containing the polylinker was identified and designated pNTEP2.
This polylinker includes the Sma I site into which the CpTI cDNA fragment was inserted.
The nucleotides downstream correspond to sequence components of the polylinker of pUC19.
The polylinker insertion region was squenced in pl8kexpHDEL.
The nucleotides downstream correspond to sequence elements of the pUC19 polylinker.
In one embodiment, the construct contains a polylinker to facilitate further subcloning.
The plasmid pZMP6 was cleaved with SmaL to linearize it at the polylinker.
The polylinker had the following sequence of restriction enzyme recognition sites,.
