Examples of 'polymerase promoter' in a sentence
Meaning of "polymerase promoter"
polymerase promoter - In molecular biology, a polymerase promoter is a region of DNA that initiates transcription by RNA polymerase. It plays a crucial role in gene expression and regulation
How to use "polymerase promoter" in a sentence
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polymerase promoter
The RNA polymerase promoter may be a hybrid promoter.
In some embodiments, oligonucleotides are used comprising an RNA polymerase promoter region.
An RNA polymerase promoter may be operably linked to the polynucleotide.
In some embodiments, oligonucelotides are provided comprising an RNA polymerase promoter region.
The RNA polymerase promoter may be single stranded or double stranded.
The most preferred promoter is one which is capable of recognizing the T7 polymerase promoter.
When present in a vector an RNA polymerase promoter maybe operably linked to the polynucleotide.
In this example SARE2 has a sequence for an RNA polymerase promoter.
Preferably, the RNA polymerase promoter is a bacteriophage promoter.
These constructs are inserted into plasmids behind a bacteriophage T7 polymerase promoter.
In addition, the promoter may be a modified RNA polymerase promoter having increased promoter activity.
A T7 polymerase promoter downstream of the mcs facilitates sequencing of DNA inserted into the mcs.
Such constructs would have the bacteriophage-specific RNA polymerase promoter sequence proximal to the indicator gene.
The method of claim 1, wherein the expression control sequence of 1 ( a ) is an RNA polymerase promoter.
In some embodiments, said RNA polymerase promoter region comprises a T7 promoter region.
See also
All the constructs are placed under the control of the T7 phage RNA polymerase promoter.
In a preferred embodiment the RNA polymerase promoter sequence is recognized by T7 RNA polymerase.
This placed the receptor sequences directly downstream from the T7 RNA polymerase promoter element.
The clone of claim 1 wherein the RNA polymerase promoter is the T7 RNA polymerase promoter.
The poliovirus cDNA is cloned behind a bacteriophage T7 RNA polymerase promoter.
One encodes the negative sense T7 polymerase promoter and the substrate sequence or the 121oxRz sequence.
The most preferred promoter is a T7 RNA polymerase promoter.
T7 RNA polymerase promoter sequence.
All APMV cDNA constructs are under the control of the T7 polymerase promoter.
The sequence from the T7 polymerase promoter to the Stu1 site of the resulting construct was,.
In some embodiments, the 5 ' portion of an oligonucleotide comprises an RNA polymerase promoter region.
The kit of claim 1, wherein said RNA polymerase promoter region comprises a T7 promoter region.
Located 5 ' to the inserted multiple cloning site is the T7 RNA polymerase promoter.
The clone also comprises an RNA polymerase promoter operatively linked to the HPIV-3 antigenome-encoding sequence.
The 5 ' - end portion also comprises bacteriophage RNA polymerase promoter region.
In addition, the downstream primer contains T7 RNA polymerase promoter sequences and the non-complementary sequences.
System according to claim 1 or 2, wherein the non-cholera toxin promoter is the T7 RNA polymerase promoter.
The resulting double-stranded DNA has a functional T7 RNA polymerase promoter recognized by the respective enzyme.
NO, 10 decoy probe containing a sequence similar, but not identical, to a T3 or T7 RNA polymerase promoter.
A vector containing the gene for RAB-7 and the T7 polymerase promoter was constructed.
The 3 ' primer additionally included the T3 RNA polymerase promoter.
Each pair contains one primer which has a T7 RNA polymerase promoter at the 5 ' end.
T7, T3 and SP6 may be listed as examples of the RNA polymerase promoter.
A typical sequence of the T7 RNA polymerase promoter is,.
This creates a 331 base pair DNA fragment carrying the T7 RNA polymerase promoter.
In certain embodiments, the T7 RNA polymerase promoter is used.
Positions of restriction sites and the 17 base T7 RNA polymerase promoter are indicated.
The resulting amplified DNA thus contained the T3 RNA polymerase promoter at the 3 ' end.
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Quantitative real time polymerase chain reaction
Polymerase chain reaction testing is done upon request
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