Examples of 'polynucleotide kinase' in a sentence
Meaning of "polynucleotide kinase"
polynucleotide kinase - an enzyme that catalyzes the transfer of phosphate groups to nucleic acids like DNA or RNA
How to use "polynucleotide kinase" in a sentence
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polynucleotide kinase
The linkers were treated with polynucleotide kinase before use.
Synthetic probes are conventionally labeled with radioactive phosphorus using polynucleotide kinase.
Restriction endonucleases and polynucleotide kinase were from New England BioLabs.
The synthetic oligonucleotide was prepared for use by reaction with ATP and polynucleotide kinase.
The fragments were treated with T4 polynucleotide kinase according to the manufacturer 's instructions.
The oligonucleotides were quantified and kinased with polynucleotide kinase and ATP.
Polynucleotide kinase treatment and subsequent re-ligation of the PCR product yielded the plasmid pCR-J1R-flanks.
The synthetic mutagenic oligonucleotide was phosphorylated at its 5 ' end using polynucleotide kinase.
The OLIGOs were phosphorylated with T4 polynucleotide kinase and then heat denatured.
The oligonucleotides can be 5 ' phosphorylated chemically during synthesis or after purification with polynucleotide kinase.
The 5 ' ends of the oligonucleotides were labelled using polynucleotide kinase as described in the literature.
After oligonucleotide synthesis, the synthetic oligonucleotide was phosphorylated at its 5 ' end using polynucleotide kinase.
Each oligonucleotide was phosphorylated using ATP and T4 polynucleotide kinase by standard procedures see, Maniatis, op.
The blunt-ended fragments can be phosphorylated by enzymatic treatment, for example using polynucleotide kinase.
The primer was labeled by use of a polynucleotide kinase and gamma-32P ATP.
See also
Following heat inactivation of the ligase, the cDNA was phosphorylated using T4 polynucleotide kinase.
The oligonucleotides preferably are treated with polynucleotide kinase, for example, T4 polynucleotide kinase.
In another preferred embodiment, the ligase is T4 and the kinase is polynucleotide kinase.
The mixed oligonucleotides are treated with T4 polynucleotide kinase to 5 ' phosphorylate the oligonucleotides.
The isolated blunt ended hY-IFN DNA is phosphorylated using polynucleotide kinase.
This agent can be, e.g., polynucleotide kinase or alkaline phosphatase.
Optimal proportions of adaptored vector and cDNA were ligated in the presence of T4 polynucleotide kinase.
This phosphorylation reaction was effected with T4 polynucleotide kinase ( manufactured by Takara Shuzo Co. ).
For use in mutagenesis reactions, the oligonucleotide was phosphorylated using T4 polynucleotide kinase.
For example, a T4 polynucleotide kinase can be used to remove the 3 ' phosphate group.
The oligodeoxynucleotides 1 and 3 are phosphorylated using ATP and polynucleotide kinase according to standard procedures.
Ten units of T4 polynucleotide kinase was added and the mixture was incubated at 37°C for 30 minutes.
The oligonucleotides were kinased using adenosine triphosphate and T4 polynucleotide kinase.
Following phosphorylation the T4 polynucleotide kinase was inactivated by heating the solution at 70°C for 10 minutes.
Preferred methods of labelling the DNA sequences are with 32P using Klenow enzyme or polynucleotide kinase.
The synthetic 14-mers were ³ ² P-labelled using polynucleotide kinase as previously described.
The single sequence oligonucleotide ( sequence shown above ) was labeled with Y32p-ATP using polynucleotide kinase.
Radioactive Y32P-ATP and T4 polynucleotide kinase were the same as in Example 6.
Each oligonucleotide was phosphorylated using T4 polynucleotide kinase.
The resultant amplicon is then phosphorylated using polynucleotide kinase ( New England Biolabs ).
Filling of ends by Klenow Polymerase and phosphorylation of the ends by T4 Polynucleotide kinase.
This PCR product wa s phosphorylated with T4 polynucleotide kinase and gel purified.
The resultant DNA ends are phosphorylated with the aid of T4 polynucleotide kinase.
Their 5 ' ends were phosphorylated by T4 polynucleotide kinase before ligation.
Oligonucleotides were labelled to a specific activity of approximately 108 dpm / µg using T4 polynucleotide kinase.
The 5 ' ends of the DNA were phosphorylated using T4 polynucleotide kinase and adenosine triposphate.
All six oligonucleotides were phosphorylated at their 5 ′ - ends with polynucleotide kinase.
The 5 ' ends of the DNA were phosphorylated using T4 polynucleotide kinase and adenosine triphosphate.
The PCR product termini were phosphorylated using T4 polynucleotide kinase.
The blunt-ended fragment was then phosphorylated using the T4 polynucleotide kinase in the presence of ATP.
These two oligonucleotides are radiolabeled with 32 p at their 5 ' - OH end by the polynucleotide kinase.
The resultant amplicon was then phosphorylated using T4 polynucleotide kinase ( New England Biolabs ).
The forward strand primer was end-labeled in the presence of 33P-YATP and polynucleotide kinase.
The 5 ' ends of the DNA were then phosphorylated using polynucleotide kinase as follows, DNA 30µl.
Oligonucleotides are usually end-labelled with Y32P-labelled ATP and polynucleotide kinase.
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This polynucleotide codes for antimicrobial activity
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Polynucleotide capable of selectively hybridizing to a polynucleotide as defined in a or b or c
Examples of using Kinase
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Blood tests show elevated creatine kinase
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