Examples of 'positive colonies' in a sentence
Meaning of "positive colonies"
positive colonies - This phrase refers to a group of microorganisms or cells that grow and multiply in a culture medium, typically used in laboratory settings for research or testing purposes
How to use "positive colonies" in a sentence
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positive colonies
Positive colonies were subcloned twice by limiting dilution.
This releases the antigen from the positive colonies.
The positive colonies were pooled for scale up.
Ten of the originally identified thirteen positive colonies were confirmed in the secondary screen.
Positive colonies were also further confirmed by sequencing.
Thirteen clones were picked from the positive colonies and grown in a liquid medium.
Positive colonies were picked and expanded in liquid culture.
A series of biochemical tests were used to confirm positive colonies enumerated on the various media.
Four positive colonies were identified using this procedure.
Minipreparations of plasmid DNA are prepared from positive colonies.
Positive colonies were identified as colonies surrounded by a blue halo.
Endoglucanase II and Endoglucanase III positive colonies were identified as colonies surrounded by a blue halo.
Positive colonies were identified through autoradiography.
Endoglucanase III and Endoglucanase IV positive colonies were indentified as colonies surrounded by a blue halo.
Positive colonies were identified via restriction enzyme digestion.
See also
Neocin ™ was used to select the positive colonies.
Positive colonies were selected with neomycin.
After secondary screening, individual positive colonies were identified.
The positive colonies were verified by sequencing.
Potential positives were taken through a final, tertiary screen to isolate single positive colonies.
Positive colonies were screened by hybridization.
Following hybridization with both olignucleotides, positive colonies were picked for DNA sequencing.
Positive colonies were selected and insert orientation confirmed by sequencing.
Plasmids were purified from 25 positive colonies and mapped by digestion with Bam HI.
The positive colonies originating from single cells were marked.
The colonies were transferred to Hybond N filters ( Amersham ) and probed for positive colonies.
Positive colonies were identified by autoradiography.
Plasmid DNA from short arm positive colonies was prepared using a Spin Miniprep Kit ( Qiagen ).
Positive colonies were scored.
Using this method 13 positive colonies were obtained.
Positive colonies are screened for beta galactosidase by filter lift assay.
After 2 to 4 rescreenings positive colonies were fermented as follows,.
Positive colonies are grown up and plasmids are analyzed by restriction endonuclease mapping.
Approximately 30 potential positive colonies were detected.
The positive colonies were further expanded and grown in large volume.
After 7 to 15 days of growth on selective medium positive colonies were counted.
Pectin methylesterase positive colonies were identified as colonies surrounded by a white halo.
Confirm only Gram-negative, oxidase positive colonies.
Positive colonies were picked and further analyzed using EcoRI restriction enzyme.
Folate levels in cultures derived from 15 positive colonies were monitored Fig.
The positive colonies were picked and the obtained vectors were subjected to sequencing.
The plasmid DNA from one of the positive colonies was designated pCFM936 apr 2.
The positive colonies exhibiting xylanase activity showed a blue diffuse halo.
A hybridization temperature of 55°C was employed and 5 positive colonies were identified out of a total of 400.
Positive colonies were identified via restriction enzyme digestion and sequencing reactions.
Cosmid DNA isolated from these positive colonies was called pPRGDHcos1, pPRGDHcos2 and pPRGDHcos3.
Positive colonies identified were grown from master plates in liquid overnight cultures.
After incubation for 1-2 days at 30°C, positive colonies were identified as colonies surrounded by a blue halo.
Positive colonies were counted relative to total colonies to give the average transposition frequency.
The hybridization filters were washed in 0.5X SSC at 65°C and positive colonies identified by autoradiography.
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