Examples of 'post transfection' in a sentence
Meaning of "post transfection"
This phrase is commonly used in molecular biology or genetic research to describe events or phenomena that occur after a genetic material has been introduced into a cell through a process called transfection
How to use "post transfection" in a sentence
Basic
Advanced
post transfection
The cell cultivation is continued for eleven days post transfection.
Two hours post transfection the cells were split and seeded into a microtiter plate.
These data describe the high levels of hGH protein produced in a few days post transfection.
Forty hours post transfection the conditioned medium was harvested and cleared by centrifugation for analysis.
CAT activity was assayed two days post transfection.
Twenty four hrs post transfection total lysates were analysed for SIVA expression.
NIK levels in the lysates were assessed thirty hours post transfection.
One strategy was to add it 6h post transfection directly in the culture.
Progress of viral infection was checked by EGFP detection at indicated times post transfection.
Twenty four hours post transfection a sample is drawn and quantified using e . g.
The cells were treated with Sodium butyrate one day post transfection to boost expression levels.
Plates were first screened for glutamine-independent transfectants at approximately 3 weeks post transfection.
The cells were harvested 48 h post transfection of the plasmids indicated in the left margin.
The medium was refreshed 6 hours post transfection.
The cells were harvested 48 hours post transfection for ligand binding or signal transduction experiments.
See also
The x-axis represents the days post transfection.
About four weeks post transfection supernatants were harvested for biochemical characterization . Example 3.
Fluorescence was detected no later then 4 hours post transfection.
Post transfection RNA samples from several time points were analyzed with Agilent 44K microarray.
The medium was changed with new differentiation medium 4 hours post transfection.
Lysis and analysis were performed 24 h post transfection using the Promega Dual Luciferase Kit.
Virus particles were harvested by collecting cell culture medium 72 hrs post transfection.
Human IgG1 was harvested or detected several days post transfection from the cell culture supernatants.
This procedure resulted in the recovery of infectious influenza virus on day 4 post transfection.
After 24 hours post transfection a medium sample was drawn.
The proteins were analyzed in the form of raw supernatants harvested 48 hours post transfection.
Cells were collected 96 hours post transfection and counted.
For isolation of transfectant viruses cells and supernatants were harvested 2 days post transfection.
Cells are assayed for activity 72 hours post transfection as detailed in examples 4 and 7.
Transient transfections were performed in triplicate and supernatant and cells were harvested 48 hours post transfection.
The cells were harvested 48 hours post transfection for analysis.
Southern blot analysis of intracellular replicative HBV DNA intermediates was performed 4 days post transfection.
To this end, cells were harvested 24 hours post transfection and subjected to lysis.
The co-transfections are harvested when cytopathic effect ( cpe ) is complete, approximately 5 days post transfection.
Lysates were analysed by immunoblot 72h and 96h post transfection with the indicated RNAi molecules.
This was expected because the first sorting round took place only 2 days post transfection.
Whole cells lysates are prepared 24 h post transfection and subjected to SDSPAGE.
Supernatants from the CHO cell cultures were collected at 48 hours post transfection.
Cells were lysed 24 h post transfection and CERT isoforms were immunoprecipitated with anti-Flag antibody.
The cells of both dishes per combination are analyzed 24 hours post transfection for luciferase activity.
Supernatants were collected 3-4 days post transfection and the antibodies were purified via protein A-sepharose chromatography.
The conditioned medium was harvested 48 hr post transfection.
Two days post transfection 3 µg / ml puromycin ( Sigma ) was added to each culture.
Tissue culture supernatants were harvested 5-6 days post transfection.
Transfected CHO-S cells were harvested 1-2 days post transfection and used in subsequent assays.
Samples were obtained from a 48-hour collection of serum containing medium at 88 hours post transfection.
Viral supernatant was collected approximately 42 hours post transfection and filtered through a 0.45 µm filter.
Samples were obtained from a 48-hour collection of serum-containing medium at 88 hours post transfection.
The transfected cells were harvested at 3 days post transfection and stored at -80 ° C until use.
In a preferred embodiment of the invention, the first harvest time is between 24h and 36h post transfection.
You'll also be interested in:
Examples of using Post
Show more
He was going to post them for the world to see
Post requirements by duty station and mission
One vacant professional post is under recruitment
Examples of using Transfection
Show more
Magnetic nanoparticle transfection is the technical term
The transfection efficiency is tested at variable times
Several methods of cell transfection exist at present
