Examples of 'post-transfection' in a sentence
Meaning of "post-transfection"
Post-transfection: refers to events or processes occurring after the introduction of foreign genetic material into a cell. It is commonly used in molecular biology and biotechnology
How to use "post-transfection" in a sentence
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post-transfection
Cells were passaged two weeks post-transfection.
Hours post-transfection immunofluorescent staining of HEK cells was performed.
Antibody is harvested from cell medium three weeks post-transfection.
Early post-transfection HCV RNA amplification was greatest for the IFN-treated cell line.
Mice were bled after two hour fasting on indicated days post-transfection.
FVIII expression at three days post-transfection was assessed by Coatest chromogenic assay Chromogenix Corp.
The media on the cells was replaced with fresh selection media at day 5 post-transfection.
Three hours post-transfection 2 ml medium was added.
Medium supernatants were harvested three days post-transfection and passaged once.
On days 4 or 5 post-transfection the cell culture supernatant was harvested.
Appropriate selection pressure was applied three days post-transfection to the transfected cells.
The transfected cells were observed under phase contrast microscope or fluorescence microscope at 48 hours post-transfection.
The effect of each vector on qP and IVC determined post-transfection are presented in Figure 6.
The cells tolerated the Stemfect RNA Transfection reagent with unmodified mRNA without a media change post-transfection.
Cells were treated post-transfection with 10 µM compound in growth medium.
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The cells were harvested 3 days post-transfection.
Briefly, 5 days post-transfection or transduction, the cells were harvested and pelleted.
Cells were harvested at approximately 48 hours post-transfection by centrifugation.
Furthermore, quantative PCR post-transfection showed an increase of wild-type mtDNA over that of mutant mtDNA.
Expression was measured 7 weeks post-transfection.
The cells were harvested 48 hours post-transfection and washed twice in phosphate buffered saline.
Two unique siRNAs were assayed in a pooled format at 72 h post-transfection.
Cells were washed 24 hours post-transfection before fresh medium was added.
The condition medium was harvested after 92 hours post-transfection.
Supernatants were harvested 72h post-transfection and purified on nickel resin.
Culture supernatants were analyzed for protein expression at 48 h post-transfection.
The plates were refed at day 5 post-transfection with fresh selection media.
Cell lysates or conditioned media were harvested at 3 or 4 days post-transfection.
The silencing efficiency was assessed 48h post-transfection by measuring the luciferase activity.
In brief, the cell culture supernatant was collected 6 days post-transfection.
The cells are assayed for gene silencing 48 hrs post-transfection by immunoblotting with respective antibodies.
In a further particular embodiment, a single harvest is carried out 48 hours post-transfection.
Sodium butyrate is added 24 hours post-transfection without changing the medium of the culture ; and.
Restoration of the dystrophin synthesis could be detected as soon as 16 hours post-transfection.
As shown in Fig 7a-b, lethal irradiation post-transfection did not inhibit transgene expression.
The cells were subjected to matrigel invasión and endothelial recruitment assays 96 hours post-transfection.
Nuclear fractions were prepared 48 hours post-transfection according to the procedure described by Mendelson et al.
The viruses were collected from the culture supermatants on days 2 and 3 post-transfection and concentrated.
Cells were analyzed 2 days post-transfection using a BD LSRII flow cytometer.
For cell invasión and endothelial recruitment in vitro assays, the cells were used 96 hours post-transfection.
Total RNA was collected 24 hours post-transfection and hHtt levels were determined by Q-PCR.
Clusters of GFP positive cells were evident by 3 days post-transfection.
Twenty-four hours post-transfection the cells were stimulated with increasing concentrations of compound 10 for 18 h.
Processing was observed by small transcript northern blot 48h post-transfection of shRNA-expression plasmids.
Seventy-two hours post-transfection the medium was removed and replaced with medium containing 0.7 % agarose.
HaCaT cells were harvested for mRNA isolation 24h post-transfection.
Samples were taken 48 and 72 hours post-transfection and were assayed using TIMP ELISA.
Total DNA was extracted from the transfected cells 48 hours post-transfection.
Total RNA was isolated at 24 h post-transfection using 1 ml of Trizol.
The level of gene knockdown was quantitated by RT-PCR analysis at 28 hours post-transfection.
