Examples of 'pre-culture' in a sentence
Meaning of "pre-culture"
A 'pre-culture' is a preliminary culture of cells or microorganisms before the main culture is started
How to use "pre-culture" in a sentence
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pre-culture
Preparation of pre-culture for production.
Pre-culture oxygenation can be carried out by agitation or by aeration.
This was designated as a pre-culture solution.
The pre-culture was used to inoculate the fermentation medium.
The culture solution was used as a pre-culture solution.
Maintenance of pre-culture and preparation of test organism for testing.
Mycological slant agar for the pre-culture of moulds.
A pre-culture medium was used to prepare the innoculum for the fermentation.
Components and protocols for the pre-culture are listed below.
Initial pre-culture shake flasks were inoculated from frozen stocks in each case.
Two liters of growth medium were inoculated with this pre-culture.
The pre-culture serving to culture the fermenter is possibly removed during a preceding fermentation.
Optionally, prior to step a the strain is grown in a pre-culture in a shake flask.
This solid pre-culture is then used to inoculate a liquid pre-culture in shake flasks.
The fermenter of the first fermentation area was seeded with 1 l of this pre-culture.
See also
Pre-culture incubation time can vary according to the strains and to other conditions of implementation.
The complete medium CgIII was used as the medium for the pre-culture.
The pre-culture can also be carried out intermittently as a continuous process with cell recycling.
The recovery rate was expressed as a percentage of live cell counts post-culture to pre-culture.
In some cases, a seed fermenter was used to provide a larger pre-culture for inoculating a production fermenter.
The pre-culture was stopped after 6h of incubation.
Alternatively, spores from frozen working stocks were inoculated directly into pre-culture medium.
Protoplast isolation Pre-culture of moss tissue for optimal protoplast isolation.
To avoid catabolite repressing effect of glucose, for the pre-culture medium with galactose was used.
In particular, the pre-culture medium has the following composition,.
The fermentation process of any one of the preceding claims wherein step b comprises a pre-culture phase.
The composition of the organic pre-culture medium used for HAOB cultivation was listed below,.
The first inoculation of Desulfonatronum lacustre was performed from a pre-culture on rich MLF medium.
In addition, the pre-culture is preferably carried out under aerobic conditions.
The production fermenter is inoculated with a pre-culture of Chlorella protothecoides.
The culture medium is inoculated with a 5 % volume of a growing pre-culture.
Preferably, also the medium used in the pre-culture is free of antibiotics.
This pre-culture was used to seed a new bacterial culture at 1 %, prepared under the same conditions.
Optionally, isoleucine is also present in the pre-culture medium.
By way of example, the pre-culture step advantageously comprises the following steps whereby,.
Modified andrews' medium for stock culture and pre-culture.
The reactor is inoculated at 1 % from the pre-culture and operated in discontinuous mode.
Data is shown relative to pGAP at the same time point . pre-culture.
Using a robotic inoculation unit, 5 µl of pre-culture was inoculated in the main culture plates.
Each main culture medium was inoculated with 30 ml of the above-mentioned pre-culture liquid.
The resulting solution of pre-culture medium is sterilized and transferred to a sterile 500 ml baffle flask.
In the alternative, a single-step fermentation without a pre-culture step is possible.
After these 8 hours a pre-culture of Lactobacillus plantarum is added.
A medium injection pipe 5 having an automatic injection valve 5a is provided with the pre-culture tank 1.
Details for preparation of a pre-culture are given in Appendix 4.
Pre-cultures, from a culture saturated with YPG, two pre-culture steps were carried out.
The inocula came from a 24 hour pre-culture in a Davis Mingioli culture medium.
The pre-culture fungus was grown on lactose to a concentration of 30 g / l.
FIG . 4, Histogram presenting the influence of pre-culture conditions on vitamin K2 production according to strain.
For the following culture, inoculation is carried out at 1 % using the pre-culture.
