Examples of 'primer concentration' in a sentence
Meaning of "primer concentration"
Primer concentration is the amount or ratio of primer molecules used in a polymerase chain reaction (PCR) or other molecular biology techniques to initiate DNA amplification
How to use "primer concentration" in a sentence
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primer concentration
Effect of primer concentration on cDNA yield.
In contrast to denaturation, annealing efficiency depends on the primer concentration.
Primer concentration should be optimized for each lot of new primer.
It is indicated that adjustment of the primer concentration may be required.
Primer concentration therefore plays a significant role in the accuracy and efficiency of RNA amplification.
Further, when using a large number of primers the total primer concentration might be an issue.
This higher primer concentration results in undesirable amplification products at a lower number of PCR cycles.
Fig . 3 shows optimization of primer concentration.
Effect of primer concentration is illustrated by Figure 4.
At 0.5 µM polymerase, virtually no amplification was seen at any level of primer concentration.
In an embodiment, increasing the primer concentration may decrease the DOR variance.
Primer concentration and volume,.
Results using samples with a 10 - fold decrease in primer concentration were dramatically different.
At these levels, primer concentration is apparently the limiting factor on yield of product DNA.
Figures 9 and 10 show the effect of first primer concentration in TRAM.
See also
The effect of the primer concentration on template - dependent RNA synthesis is shown in Table 1.
An internal reference is used, such as amplification of the 28S rRNA with limiting primer concentration.
Primer concentration is adjusted using autoclaved TE buffer to 20 um.
Figures 11 and 12 show the effect of second primer concentration in TRAM.
Effect of primer concentration is illustrated by FIG . 4.
Primer concentration in the final PCR reaction was 1 micromolar except for GAPDH which was 0.5 micromolar.
Increasing the 2150 primer concentration to 4µM did not markedly increase product yield.
A primer concentration of 10µM is obtained with a final volume of 50 µl.
The effect of RT primer concentration was analyzed ( Figure 3A ).
Nested primer concentration may generally be between 5-50 pmol.
Consequently, as loop primer concentration increased, acceleration of the LAMP reaction was observed ( Fig . 7 ).
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