Examples of 'primer extension' in a sentence

Meaning of "primer extension"

Primer extension is a technique used in molecular biology and genetics to determine the starting point or sequence of DNA or RNA molecules. It involves using a primer, a short piece of DNA or RNA, that binds to the target molecule and serves as a starting point for DNA synthesis. By extending the primer, researchers can identify and analyze specific genetic sequences

How to use "primer extension" in a sentence

Basic
Advanced
primer extension
The primer extension itself is not copied.
Dashed lines represent primer extension products.
The primer extension includes an endonuclease restriction site.
Primer sequence for primer extension systems.
Primer extension occurs until a complementary dideoxynucleotide is incorporated.
Polymerase activity was determined by primer extension reactions.
Primer extension and hybridisation to array.
Performing at least a first primer extension reaction.
The primer extension method is used in a number of assay formats.
The result is a primer extension product.
A container providing a buffer suitable for primer extension.
Another example for primer extension is nick translation.
The primer extension reagent module is manufactured by injection molding.
This is required for primer extension.
Single primer extension abr.

See also

Conducting the amplification reaction to form primer extension products.
A first primer extension product may be generated with a polymerase.
This direction is confirmed by the results of a primer extension experiment.
Primer extension products are formed by polymerization of deoxynucleoside triphosphates by a polymerase.
Conditions suitable for primer extension are known in the art.
No primer extension products were obtained in the yeast tRNA control reactions.
The present invention relates to a primer extension reaction method.
A second primer extension product may be generated with a polymerase.
Detecting the lengths of the primer extension products in the pool.
Any primer extension products are then amplified using a further primer.
The degradation of enriching primer extension product gives fluorescence signal.
The primer extension reagent module may have different designs.
Kits are provided for use in primer extension methods described herein.
The primer extension reaction is performed using any suitable method.
This results in inefficient primer extension and poor amplification efficiency.
The primer extension reaction is also useful in the present invention.
An oligonucleotide containing the desired mutation is used for primer extension.
Approaches using primer extension and detection by fluorescence have been shown.
Such a temperature range will normally be maintained during primer extension.
The synthesized primer extension product is complementary to the target sequence.
A perfect complementarity is not required for the primer extension to occur.
Examples of extension moieties of primer extension units are deoxynucleotides or dideoxynucleotides or their analogs.
An amplification primer is a primer for amplification of a target sequence by primer extension.
Reaction conditions suitable for primer extension reactions are known in the art.
In that case the probe would be digested concomitant to primer extension.
Quantitative primer extension assay can be used for quantification of globin mRNA.
One or more of the nucleotides used in primer extension may be labelled.
Use of a primer extension reaction also can be applied in methods of the technology.
Arrows indicate position of transcription initiation site by primer extension analysis.
A detailed description of a cycle of a primer extension chain reaction of the present invention follows.
Here two different immobilised primers are used to provide primer extension.
The separation moiety of the primer extension unit is capable of attaching to a solid support.
The present invention provides methods of performing a primer extension reaction.
The amplification is carried out under primer extension conditions at essentially constant temperature.
There is no requirement to use a thermostable polymerase for a single primer extension reaction.

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