Examples of 'primer pair' in a sentence
Meaning of "primer pair"
primer pair: A primer pair refers to a set of two short, synthetic, single-stranded DNA sequences that are used in molecular biology techniques like polymerase chain reaction (PCR) for the amplification of specific DNA fragments
How to use "primer pair" in a sentence
Basic
Advanced
primer pair
A primer pair having a region desirably inserted is prepared.
No template controls were set for each primer pair.
The universal primer pair was used for normalization.
Magnesium concentration and pH were optimized for each primer pair.
A primer pair need not be of the same length.
Optimal primer concentrations were determined for each primer pair.
The amplification primer pair is an amplification pair disclosed herein.
The same strategy was used to select the other primer pair.
A control reaction for each primer pair minus template was included.
A primer pair comprising such a primer is still considered to span an intron.
Mutations not observed using the new primer pair were excluded.
A conserved primer pair can be synthesized according to the pol gene sequences.
A control reaction minus template was also performed for each primer pair.
The use of the bivalent primer pair therefore gives unspecific bands.
Reactions with water instead of cDNA template were run with each primer pair as control.
See also
The amplification primer pair can be an amplification primer pair of the invention.
A standard curve is generated for each primer pair using control cRNA.
The universal primer pair includes a forward primer and optionally a reverse primer.
The conditions are optimized for each particular primer pair according to standard procedures.
This same primer pair also amplifies the internal quantification standard sequence.
Individual clones obtained using a selected primer pair were aligned to yield a consensus sequence.
This primer pair only produces an amplicon if the phage genome recircularises upon excision.
Tubes or wells can be prepared with a primer pair for a single gene to be measured.
Cloning of all genes into their respective vectors was done using the applicable primer pair.
The distance between a primer pair is reflected by the size of the amplified sequence.
Two consecutive amplification reactions take place with a different primer pair used in each reaction.
One of the primers of a primer pair can be longer than the other primer.
Also included in the reaction is a conventional reverse primer to create a PCR primer pair.
This primer pair is preferably used in an enzymic amplification reaction such as PCR.
MLPA facilitates the amplification and detection of multiple targets with a single primer pair.
This primer pair was used in PCR under a variety of conditions to optimize amplification.
Then RPA is performed using the primer pair and the RPA product is analyzed.
The second primer pair defines a DNA sequence which is a portion of the first amplified sequence.
Expected product size for Primer Pair.
PCR with this primer pair will therefore amplify and facilitate identification of the intervening sequences.
A gene amplified using a primer pair of SEQ ID Nos.
The primer pair generates a unique PCR product.
In one embodiment, a single primer pair is used.
The following primer pair was used for amplification of Fusarium genomic DNA.
A non-RT control was performed for every primer pair.
A universal primer pair amplifying deaminated CMV promoter DNA irrespective of the methylation status.
The amount of non-specific amplification is primer pair dependent.
The primer pair is of the following composition,.
In some embodiments, the kits may comprise a universal primer pair.
The primer pair employed was as follows,.
Estimated band sizes for each primer pair are given below,.
A with the primer pair of the following composition,.
In certain embodiments, the at least one primer comprises a primer pair.
At least one primer pair selected from the group consisting of,.
On occasion, extra fragments can be eliminated by labeling the opposite primer in a primer pair.
