Examples of 'primer set' in a sentence
Meaning of "primer set"
A primer set typically refers to a collection of short DNA sequences, known as primers, that are used in the polymerase chain reaction (PCR) technique to amplify specific regions of DNA. The primer set consists of two primers, one for each end of the target DNA sequence, and is crucial in initiating the DNA amplification process
How to use "primer set" in a sentence
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primer set
Amplification was optimized for each primer set.
The primer set used are as follows.
In this way only one primer set is required.
The sequencing reactions were performed using the primer set.
For each primer set two batches were tested.
Sebastian in the presence of primer set no.
For each primer set a standard line was included in the experiment.
Prestige in the presence of primer set no.
The specificity of each primer set was monitored by analysing the dissociation curve.
Buffer and cycling conditions were specific to each primer set.
Each graph is labeled with the primer set that was employed.
Each primer set was run in separate reactions and each sample was run in triplicate.
In the present invention such a primer set is termed a nested primer set.
The primer set consists of one first primer and two second primers.
Thus selected plasmids were sujected to sequence analysis using the above primer set.
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The primer set can be used for amplifying and detecting a polynucleotide to be detected.
The above result also confirmed that the primer set was working in a specific manner.
The primer set did not amplify a diagnostic fragment from healthy banana leaf tissue.
The size of the amplified fragment obtained with each primer set is also shown.
Amplifications with each primer set and input target number were carried out in triplicate.
The amplification is the average amplification efficiency for all samples for a particular primer set.
For the amplification a primer set is provided wherein one of the primers contains a label.
Contacting a sample comprising cotton DNA with a primer set and a probe set that.
A first primer set represents a first target sequence and a second primer set represents a second target sequence.
These data demonstrate that the primer set developed by Hiramatsu et al.
The primer set was found to have high specificity only to Slackia sp.
Using this primer set of SEQ.
In this way the central region ( the barcode region ) can be amplified by a primer set.
A second primer set was used for the confirmation of HGV positive samples.
In some embodiments, the third primer of the second primer set comprises a target specific sequence.
A new primer set P was subsequently tested on several será.
An example of such a primer set is shown in Figure 1.
Primer set 2 was the same set used to generate probe for the cDNA library screening.
A standard curve of each primer set was generated using mouse genomic DNA.
The reagent can be a nucleic acid, including an oligonucleotide or an RT PCR primer set.
A commonly used PCR primer set first published by Sullivan et al.
Optimizations ( primer concentration and temperature ) for Pol primer set were carried out.
The primer set used for RACE is as follows,.
The assay may quickly and conveniently be performed using only primer set ( A ) above.
The optimal primer set described in Example 2 was utilized in this experiment.
The DNA encoding mGluR4 was isolated from a rat cDNA using the primer set.
This primer set amplifies crHIV RNA from a crHIV-specific polylinker sequence.
PCR is performed using a primary primer set as described in reference to Figures 1A.
For each primer set and MgCl2 concentration, three different temperature profiles for PCR were investigated.
PCR is performed using a primary primer set as described in reference to FIG . 1A.
A common primer set is available for detection of all Trypanozoon taxa, including the three.
A DNA detection kit comprising the DNA primer set of claim 7.
The primer or primer set can be designed to amplify one or more V-segment families.
Initial testing of the group O isolate with primer set 1 showed mixed results.
The members of each primer set are co-linearly arranged as shown diagrammatically in the Figure.
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The primer includes the following modules
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