Examples of 'producer cells' in a sentence
Meaning of "producer cells"
producer cells: This phrase typically refers to cells in living organisms that are responsible for producing specific substances or components. It is commonly used in biology and medical contexts to describe the function of certain cells
How to use "producer cells" in a sentence
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producer cells
Sorting and generation of high producer cells.
All producer cells were transfected with high efficiency.
There are two common procedures for generating producer cells.
The producer cells are then cultured to produce rAAV.
Poliovirus and producer cells.
Producer cells for replication competent retroviral vectors.
The transduced cells are referred to herein as virus producer cells.
The producer cells are transfected with a viral vector of interest.
Supernatant collection can continue as long as producer cells are healthy.
Injection of producer cells would then provide a continuous source of vector particles.
The system eliminates safety risks of feline producer cells.
According to the present invention the producer cells are cultured in a perfusion system.
Immunostaining of VIPs and virions in supernatants from induced producer cells.
The producer cells are drawn up into a syringe and administered to the patient.
We employed MSCs as producer cells.
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The virus producer cells of the present invention are preferably transduced cells.
Infection was repeated twice with fresh supernatant of the same producer cells.
In preferred embodiments the producer cells are lysed by means of detergent lysis or autolysis.
Producer cells derived from human amphotropic and xenotropic packaging cell lines.
Selection of suitably altered producer cells may be conducted by any technique in the art.
Eukaryotic cells suitable for use in the present invention include producer cells.
The invention further provides producer cells prepared by the process of the invention.
Evaluation of the reporter molecules for sorting high POI producer cells.
Adenovirus and producer cells The invention relates to purification of adenovirus.
One embodiment of the invention involves infection of a producer cells with two different rHSV vectors.
Producer cells are preferably human or non-human eukaryotic cells.
INTRODUCTION The present invention relates to a means for implanting producer cells into the mammalian brain.
Culturing producer cells in a first bioreactor in suspension with a perfusion system ;.
In a particular embodiment, described herein, producer cells were transplanted into the brains of rats.
Producer cells are generally mammalian cells but can be, for example, insect cells.
In the process of the invention, producer cells are transfected with a viral vector of interest.
Producer cells are cultured to increase cell and virus numbers and / or virus titers.
Thus, the method is much more sensitive to signals generated by mutant producer cells.
The producer cells can be cultured in conventional fermentation bioreactors, flasks, and petri plates.
In this regard, the vectors may be introduced into retroviral producer cells for amphotrophic packaging.
In one embodiment, producer cells are grown under pH conditions that promote viral particle release.
This transient virus production was used to infect TEFLY-A producer cells.
In the context of the invention, producer cells are preferably human or non-human eukaryotic cells.
These results confirm the importance of the protease mutation in producing high-level Gag / Pol producer cells.
The virus / cytokine producer cells are designed to express gene products in the lymphoid organs.
Approximately 10 ml of media was harvested from a 10 cm plate of confluent producer cells.
Such producer cells are different from patients cells and may even be of non-human origin.
Infection In the methods of the invention, producer cells are infected with recombinant adenovirus.
For example, producer cells containing the retroviral vector plasmid can be cultured and supernatant collected.
Yields are given as vector genome copies per 150 mm dish of producer cells.
Once transfected, producer cells can create custom miRNA-bearing exosomes for an extended period of time.
However, 293 cells are subject to severe limitations as producer cells for adenovirus vectors.
For example, the producer cells may include a retroviral plasmid vector including a negative selectable marker.
Alternatively, when practicable, the target cells can be co-cultured with the producer cells.
Virus was isolated from the producer cells by 3 cycles of flash-freeze / thawing.
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