Examples of 'pullulanase' in a sentence
Meaning of "pullulanase"
Pullulanase is an enzyme that breaks down pullulan, a polysaccharide made up of maltotriose units, into maltotriose
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- Any of a class of enzyme that catalyze the hydrolysis and transglycosylation of α-1,4 or α-1,6 linkages in polysaccharides such as pullulan.
How to use "pullulanase" in a sentence
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pullulanase
The pullulanase activity is measured in each fraction.
A second method is used to analyse the pullulanase.
Further utilization of pullulanase is expected in these fields.
The disbranching enzyme is preferably isoamylase or pullulanase.
The pullulanase is formulated according to the intended uses.
Saccharification was performed using a glucoamylase and a pullulanase.
The pullulanase can also be dried by spraying or lyophilization.
Determination of the amino acid sequence of the pullulanase.
The pullulanase can be used for liquefaction of starch.
A composition of the invention may further comprise a pullulanase.
Pullulanase activity may be determined relative to a pullulan substrate.
Starch and amylose are almost unattacked by the pullulanase.
A pullulanase may be present during liquefaction.
The composition of the invention optionally comprises a pullulanase.
Development of the pullulanase activity indicates the same result.
See also
The process applies an expressly selected pullulanase activity.
Pullulanase is well known as a debranching enzyme of pullulan and starch.
This may in particular be the case for pullulanase.
The alkaline pullulanase can advantageously be used as a detergent component.
Thus the enzyme may be a pullulanase.
Any pullulanase may be used in a process of the present invention.
We have isolated a microorganism producing a novel pullulanase of surprising thermostability.
The pullulanase of the present invention preferably hydrolyses potato amylopectin.
All samples were added an identical enzyme mix with no pullulanase.
An alkaline pullulanase herein denotes that having an optimum pH at an alkaline range.
Depolymerisation with pullulanase.
The pullulanase can also be used to form tetraholosides starting from maltose.
In an embodiment the pullulanse is a thermostable pullulanase added during liquefaction step i.
Pullulanase and amylases are enzymes fundamental to the brewing and baking industries.
Another example is the use of pullulanase for hydrolysis of pullulan into maltotriose.
The pullulanase of the present invention allows a high starch conversion level to be achieved.
Preparation of pullulanase.
The present invention preferably relates to an enzymatic composition comprising a glucoamylase and a pullulanase.
The invention relates to the use of the pullulanase for saccharification of liquefied starch.
Furthermore the DPn fraction is halved due to the action of the immobilised pullulanase.
The techniques for harvesting the pullulanase produced are well known to the expert.
The pullulanase according to the invention is heat stable and active in a wide temperature range.
A preferable method for producing the alkaline pullulanase of this invention is now discussed.
The pullulanase according to the invention is particularly suitable for treatment of starch and pullulane.
The present invention also relates to enzymatic compositions comprising the pullulanase according to the invention.
This demonstrates that the pullulanase of the present invention has a pullulanase activity and a debranching activity.
In another aspect the composition comprises the variant glucoamylase of the invention combined with a pullulanase.
An alkaline pullulanase producing microorganism which is named as Bacillus sp.
Enzymological characteristics of alkaline pullulanase B are explained below.
The pullulanase activity was measured by a modified method of Lappalainen et al.
Enzymological characteristics of the alkaline pullulanase A are described below.
Most preferably the pullulanase is derived from Bacillus acidopullulyticus.
A preferable method for purifying alkaline pullulanase B is discussed below.
Pullulanase activity may be determined as NPUN.
The crude enzyme was thus purified to obtain alkaline pullulanase A.
