Examples of 'purified plasmid' in a sentence
Meaning of "purified plasmid"
purified plasmid: This phrase is used in biology to refer to a plasmid DNA molecule that has been separated and cleansed from other cellular components or impurities for research or experimental purposes
How to use "purified plasmid" in a sentence
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purified plasmid
The purified plasmid was digested with HindIII.
Chemically competent bacteria were transformed with purified plasmid and cultured overnight as before.
The purified plasmid was cleaved with a restriction enzyme PstI.
Protoplasts were isolated and transfected with the purified plasmid DNA using standard procedures.
The purified plasmid was cleaved with a restriction enzyme Pstl.
Transformation typically involves incubation of recipient cells with purified plasmid DNA isolated from E. coli.
Purified plasmid DNA from single colonies was purified and sequenced.
At that time, a large scale, highly purified plasmid prep is performed.
The purified plasmid was then used directly for DNA sequencing.
Sub-confluent COS-7 cells were transfected with the purified plasmid DNA by electroporation.
Purified plasmid DNA is is injected into pronuclei of fertilized rat oocytes.
Preparation of a solution for the purified plasmid DNA pCMV-VEGF165.
Purified plasmid DNA can be prepared for injection using a variety of formulations.
A solution of the purified plasmid DNA was collected into a glass container.
Purified plasmid DNA was assayed for bacterial LPS contamination.
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The concentration of the purified plasmid DNA was determined in a UV spectrophotometer and fluorometer.
Purified plasmid DNA was prepared by using commercially available Qiagen DNA purification columns.
Cells were transfected with purified plasmid DNA using a standard calcium phosphate precipitation method J.
The purified plasmid DNA was cleaved by the restriction endonucleases NheI and BamHI.
Before transfection the purified plasmid were sterilized by extraction with phenol / chloroform followed by ethanol precipitation.
The purified plasmid DNA was also checked for integrity by agarose gel electrophoresis.
Thus, twenty purified plasmid DNA were generated for both Vh and Vi.
This purified plasmid DNA was then used for DNA sequence analysis.
Thirty µg of purified plasmid was digested with SpeI for 1 hr at 37°C.
Purified plasmid DNA were diluted in a tris-HCl solution to generate a standard curve.
Plasmid DNA, Purified plasmid DNA of interest ( see above ).
Purified plasmid DNA was treated with restriction endonucleases according to manufacturer 's instructions.
The purified plasmid DNA was pooled.
Purified plasmid DNA from positive clones was further cleaned up using Wizard ™ λ preps.
Further the purified plasmid DNA may be concentrated by ultrafiltration and / or diafiltration.
Purified plasmid was then concentrated and desalted by UF / DF, and sterilized by filtration.
Ligate the purified plasmid fragment obtained in step 15 with the adapter obtained in step 16.
Purified plasmid from this E. coli strain is utilized to transform plants.
A CsCl purified plasmid DNA preparation was made from one of these clones.
This purified plasmid DNA was the used for DNA sequence analysis.
The purified plasmid DNA for the clone was designated Bip-Topo.
The purified plasmid DNA for Bip-Topo was digested with NdeI and XhoI.
CsCl2 purified plasmid DNA from subclone cCOLR6A was then mapped using a variety restriction endonucleases.
This purified plasmid DNA is then analyzed by restriction mapping and / or DNA sequencing.
Purified plasmid DNA was sequenced in both directions using M13 forward and reverse primers.
Purified plasmid was inoculated into Balb / c mice by intra-muscular injection.
Purified plasmid DNA was used to transform the expression strain BLR ( DE3 ) pLysS.
Purified plasmid DNA of the SV40 vector pSL-PL was digested with Clal and BamHl.
Purified plasmid DNA then was re-transfected into COS-7 cells for further rounds of sorting.
The purified plasmid DNAs were then used for sequencing from both 5 ' and 3 ' ends.
Purified plasmid f can be introduced into mammalian cells [ 134 ] for heterologous Ig antibody expression.
The purified plasmid preparations from these cultures constitute the pIJ922 - S . avermitilis genomic library.
The purified plasmid DNA was resuspended in 50 µL of sterile H2O.
Purified plasmid DNA was aliquoted to microcentrifuge tubes and stored at -20°C.
The purified plasmid DNA is resuspended in double-distilled water and stored at +4 ° C.
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