Examples of 'puromycin' in a sentence
Meaning of "puromycin"
Puromycin: An antibiotic drug used in molecular biology to inhibit protein synthesis
Show more definitions
- An antibiotic that is a strong inhibitor of protein translation.
How to use "puromycin" in a sentence
Basic
Advanced
puromycin
Puromycin also did not perturb efficient RNAi.
They were then selected in the presence of puromycin.
Puromycin resistant cells were cloned by limiting dilution.
Luciferase activity was assayed in puromycin resistant clones.
Puromycin resistant stable cell line was selected.
Exemplary selectable markers are hygromycin or puromycin resistance.
Puromycin is an antibiotic that acts by terminating peptide elongation.
Tre expressing cells are sensitive to puromycin.
Puromycin selection was maintained throughout culturing.
Resistant clones were selected in the medium containing puromycin.
Puromycin resistant clones are selected in the presence of tetracycline.
Transfected cells were selected by puromycin resistance.
Puromycin is an inhibitor of ribosomal translation.
The drug resistance gene may be a puromycin resistance gene.
Puromycin was less effective in inhibiting growth and protein biosynthesis.
See also
The cells were then selected in puromycin for one week.
Puromycin resistant cells are selected.
The plasmid contains the selectable marker for puromycin resistance.
Puromycin resistant clones are selected.
The plasmid also contained hygromycin and puromycin resistance genes.
Puromycin is used in cell biology as a selective agent in cell culture systems.
The transfected cells were then selected in puromycin until colonies formed.
Colonies were selected by growing transfected cells in the presence of neomycin or puromycin.
Clones are selected for their puromycin resistance and studied as described above.
The establishment of stably transduced cell lines is achieved by puromycin selection.
This result confirms the inhibition of puromycin reactivity in the presence of sparsomycin.
From cloning onward the culture media did not contain puromycin.
The puromycin resistance gene was removed using standard molecular biology techniques.
The retrovirus vector pCMSVpuro comprises a puromycin resistance gene as a selection marker.
Puromycin is an antibiotic protein synthesis inhibitor which causes premature chain termination during translation.
The sensitivity of the enzyme towards puromycin and leucine hydroxamate was also determined.
A puromycin selection marker was included in the vector for the generation of stable cell lines.
The plasmid also includes the puromycin gene for selection in eukaryotic cells.
The cells were then transferred into selective medium supplemented with puromycin and methotrexate.
Infected cells were selected with puromycin and experiments were carried out on derived cell populations.
The compound treatment and culture were continued for additional ten days before puromycin was added.
The species that forms the adduct is preferably puromycin or pyrazolopyrimidine or any related derivatives.
Puromycin selection allows identification of ES cells with insertion of the cassette.
The eukaryotic selectable marker is a puromycin resistance gene expressed from a mouse phosphoglycerate kinase promoter.
That P site binding of each tRNA had occurred was confirmed by the addition of puromycin.
GFP was cloned in to replace puromycin as the selection marker.
Human rhabdomyosarcoma cells were transiently transfected with Vpr expression plasmids and selected with puromycin.
Two genes in Streptomyces alboniger puromycin biosynthesis pathway are closely linked.
Other strategies involve using external oligo templates to align the RNA and puromycin linker.
Flow cytometry of the puromycin resistant population showed an absence of GFP expression.
In some embodiments, the peptide acceptor in this complex is puromycin.
Following transfection cells were selected in puromycin and genomic DNA was prepared.
After puromycin selection, the puromycin resistant clones were counted.
All transfected pools were selected using puromycin at a final concentration of 3 mg / ml.
Puromycin has a structure similar to that of the tyrosinyl aminoacyl-tRNA.
