Examples of 'pyrosequencing' in a sentence
Meaning of "pyrosequencing"
Pyrosequencing is a method of DNA sequencing that relies on the detection of pyrophosphate release upon nucleotide incorporation during DNA synthesis
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- A technique used to sequence DNA using chemiluminescent enzymatic reactions.
How to use "pyrosequencing" in a sentence
Basic
Advanced
pyrosequencing
Pyrosequencing requires that ssDNA template is present.
Preferably the nucleic acid sequencing is pyrosequencing.
Pyrosequencing is used mainly.
The sequencing method preferably employed is pyrosequencing.
Pyrosequencing was used to specify the bacterial taxonomy in spoiled meat samples.
The populations were identified using pyrosequencing.
In the pyrosequencing reaction the PPi is quantified using an enzyme cascade which generates light.
This region was analyzed using pyrosequencing.
Pyrosequencing is performed on each DNA fragment in parallel.
The enriched target sequences are then subjected to pyrosequencing.
Commercial equipment which utilise pyrosequencing have also been developed.
Preferably the sequencing of a nucleic acid strand is pyrosequencing.
Detailed example of the pyrosequencing data for Clostridium sp.
Sequencing can be performed by pyrosequencing.
Detailed example of the pyrosequencing data for Clastridium sp.
See also
Reagents are then added into the wells to carry out pyrosequencing.
Pyrosequencing of Mycobacterium tuberculosis extracted using SiQAC from sputum samples.
Then each amplicon is sequenced with the pyrosequencing technique.
Pyrosequencing Preferably the nucleic acid sequencing method employed is pyrosequencing.
Preferably the assay is pyrosequencing.
Pyrosequencing method is based on detecting the activity of DNA polymerase with another chemiluminescent enzyme.
The present invention will now be explained in the context of pyrosequencing.
The present sequencing method intends to improve the pyrosequencing as well as thermal cycle sequencing.
This limitation is shared by other techniques that detect single nucleotide additions such as pyrosequencing.
Incorporation of the correct nucleotide will generate a pyrosequencing signal which can be detected.
Pyrosequencing of bisulfite treated DNA.
Excitation radiation sources used for fluorescence based detection systems are not necessary for pyrosequencing procedures.
He is most famous for developing the pyrosequencing method for DNA sequencing.
Recently, pyrosequencing has emerged as a useful sequencing methodology.
Another example of method detection is the pyrosequencing technique as described by Winge Innov.
Pyrosequencing advantageously provides sequence information rapidly, typically within minutes.
Different sequencing primers were designed to carry out KRAS gene pyrosequencing analysis.
One method is the Pyrosequencing technique as described by Winge Innov.
In some embodiments, sequencing comprises pyrosequencing.
Another method is the Pyrosequencing technique as described by Winge Innov.
In this embodiment, the enriched target sequences are then subjected to pyrosequencing.
Recent studies use shotgun Sanger sequencing or pyrosequencing to recover the sequences of the reads.
Pyrosequencing exploits the release of pyrophosphate ( PPi ) upon nucleotide addition.
Beside this, there are other aspects of pyrosequencing that still need improvement.
Pyrosequencing makes use of pyrophosphate ( PPi ) which is released upon nucleotide addition.
The method of claim 1, wherein generating the sequence information comprises pyrosequencing.
Alternatively, pyrosequencing techniques may be employed.
A method according to any preceding claim, wherein the sequences are determined by pyrosequencing.
Is particularly useful for the pyrosequencing methodology, and the sequential contacting step b.
A suitable detector 8 is also provided to detect light produced by the pyrosequencing reaction.
When pyrosequencing is performed according to the dispensation order, the results shown in FIG.
The method of claim 9, wherein said processing comprises adding pyrosequencing reagents.
Massively parallel pyrosequencing reactions were performed directly on PCR products as described in section 4D.
Figure 9 depicts a double-ended sequencing demonstration on a pyrosequencing apparatus according to the invention.
After addition of enzyme-carrying beads, the sequence of the fragments is determined using pyrosequencing.
