Examples of 'quenching agent' in a sentence
Meaning of "quenching agent"
quenching agent - a substance used to regulate the cooling process of metals during heat treatment to achieve desired mechanical properties
How to use "quenching agent" in a sentence
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quenching agent
A quenching agent is added to stop the reaction.
The polymerization is partially quenched or terminated with a quenching agent.
Preferably the quenching agent is a fluorescent rhodamine or cyanine.
The polymer will continue to replicate until a quenching agent is presented.
An exemplary quenching agent of this type is a sulfonic acid.
A solution to this problem of undesired degradation was to add more quenching agent.
An example of a known quenching agent contains an organic polymer.
The absorbent material can be impregnated with the quenching agent.
The quenching agent may be incorporated in liquid or solid form.
The resultant mixture is quenched with a quenching agent and water.
The aprotic quenching agent is added until the reducing agent disappears.
The alkoxyalkyl substituted tosyloxymethylphosphonate is quenched with a quenching agent and water.
The quenching agent employed according to the invention can be any proton donor.
The fluorescent dye is therefore no longer in proximity to the quenching agent and fluoresces.
A quenching agent is added and the products are analyzed suing denaturing gel electrophoresis.
See also
The polymers of the invention are subsequently quenched with an alcohol or other quenching agent.
The amount of quenching agent used is an amount sufficient to end the polymerization reaction.
One can also use both a fluorophore and quenching agent to label the probe.
This removal of the quenching agent allows fluoresence from the reporter dye to be detected.
The polymerization reaction used to prepare the polymer nanoparticles may be terminated with a quenching agent.
For example, a quenching agent containing an organic polymer is known.
A composition as claimed in claim 1 wherein the quenching agent is cationic.
Optionally, a quenching agent may be employed to provide terminal functionality.
In a preferred embodiment, an oligonucleotide contains both a fluorophore and a quenching agent.
In a different embodiment, the quenching agent of the probe is cleaved.
Another quenching agent can be used, for example a synthetic agent.
For example, useful techniques include the addition of a protonating or quenching agent.
In particular embodiments, the quenching agent includes a polyhydroxy compound.
For instance, molecular beacons typically comprise a fluorophore and a quenching agent.
For example, a protic quenching agent may be employed to give a monofunctional polymer chain.
For example, in such systems, the reaction is typically stopped using a quenching agent.
The quenching agent may be a free amino acid, such as glycine, cysteine or lysine.
In a preferred embodiment, an oligonucleotide of the invention contains both a fluorophore and a quenching agent.
For example, a suitable quenching agent may be a base such as sodium hydroxide or potassium carbonate.
Example 3C illustrates practice of the present invention where a ketone is the quenching agent.
The quenching agent forms a water soluble adduct with the cross-linking agent.
Linear alkyl benzene sulfonate, formed as a reaction product from LAB used as a quenching agent.
In one embodiment, the quenching agent used as a reaction inhibitor is vitamin C.
Example 3A illustrates practice of the present invention where the quenching agent is a carboxylic acid.
In a preferred case the quenching agent reduces an interfering optical signal by about 99 %.
The method of embodiment 17, wherein the suitable quenching agent is sodium bicarbonate.
TaqMan based assays use a fluorogenic oligonucleotide probe that contains a 5 ' fluorescent dye and a 3 ' quenching agent.
The method of claim 1, wherein the quenching agent is a functionalizing agent.
The polymerization is allowed to proceed, and is then partially quenched ( terminated ) with a quenching agent.
A process according to claim 16, where the quenching agent is sodium or potassium thiosulfate.
APC was isolated and processed using acetic acid ( AcOH ) as a quenching agent.
The nozzles 4 are adjustable so that the quenching agent 2 hits the product 1 tangentially.
The quenching agent can also be a non-fluorescent compound or dye as dabcyl ( " Dark quencher " ).
In another embodiment, the quenching agent is L-cysteine.
In more preferred cases the quenching agent reduces optical interference by at least about 99.9 %.
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