Examples of 'racemase' in a sentence
Meaning of "racemase"
Racemase: (noun) Racemase is an enzyme that catalyzes the conversion of one enantiomer into another. It is commonly found in biological systems where it plays a role in racemization reactions
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- Any enzyme that catalyzes the stereochemical inversion of the configuration about an asymmetric carbon atom in a substrate having only one center of asymmetry.
How to use "racemase" in a sentence
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racemase
The mutant racemase was tested on several substrates.
Negative controls are set up without alanine racemase.
The purified racemase was tested in several assays.
Energetics and mechanism of proline racemase.
The commercial enzyme showed racemase activity on both isomers.
Negative controls did not contain racemase.
Use of alanine racemase genes to confer nematode resistance to plants.
Several revertants of the mutagenized alanine racemase were created.
The potential tryptophan racemase is then purified by affinity chromatography.
The activity is also known as racemase.
Controls with no racemase were included.
The systematic name of this enzyme class is alanine racemase.
The tryptophan racemase activity is purified using conventional column chromatography.
Epimerase and racemase.
A hydantoin racemase can also be used in addition to the hydantoinase and carbamoylase.
See also
Clones that are determined to contain a tryptophan racemase gene are further analyzed.
A no racemase negative control was utilized again and all samples were run in duplicate.
The invention relates to isolated polypeptides with hydantoin racemase activity.
The tryptophan racemase from other tissue sources is isolated in a similar fashion.
This disclosure describes several methods for obtaining an active tryptophan racemase.
Cell extracts were assayed for glutamate racemase activity using the following protocol.
Several combinations of the mutations were created and tested for tryptophan racemase activity.
Suitable organisms as a source for the acetoin racemase according to the invention are Lactobacilli.
The Penicillium transformants of the invention also express racemase activity.
Tryptophan racemase may play a role in Sclerochiton ilicifolius in the native monatin production pathway.
The bivalve mollusk Scapharca brouhtonii also contains an aspartate racemase.
Concentration of D-Aspartate racemase in various rat tissues.
In another embodiment, said metabolic enzyme is an alanine racemase enzyme.
Wild-type alanine racemase was purified as described above.
No dehydratase was identified, nor do the authors explain the role of the racemase.
Also the carbamoylase and hydantoin racemase may be immobilized, too.
The mutated racemase appears to retain activity on the original substrate, alanine.
A whole cell catalyst according to claim 25 which further comprises a gene encoding for a racemase.
The racemase enzyme works in both directions and retains wild-type activity.
The completed vector, therefore, contains the entire coding region of the racemase gene.
Polypeptides with hydantoin racemase activity, also called hydantoin racemases, are known in the art.
The metabolic gene may be a D-alanine racemase gene.
Further, amino acid racemase with activity toward the amino acid in question should be absent.
A basidiomycetous mushroom, Lentinus edodes, also contains a broad activity alanine racemase.
And Pseudomonas striata, proline racemase, and multifunctional phenylalanine racemase.
DNA sequencing revealed which vector encoded the S. clavuligerus racemase.
In one embodiment, the D-alanine racemase metabolic gene is expressed under an inducible promoter.
This result shows that it is possible to produce R, R monatin using alanine racemase.
Alanine racemase is PIP dependent and has been cloned from Salmonella typhimurium ( dadB gene ).
Instead, the present invention comprises DNA compounds that encode the racemase activity of Streptomyces clavuligerus.
Glutamate racemase performs the additional function of gyrase inhibition, preventing gyrase from binding to DNA.
The new construct is designated pET30Trp racemase.
When purified glutamate racemase was used, approximately 64 µg was used per 1 mL reaction.
In another embodiment, the metabolic enzyme is an alanine racemase ( dal ) enzyme.
The mutagenized racemase was cloned into pET30 for expression and purification.
