Examples of 'random priming' in a sentence
Meaning of "random priming"
Random priming: A technique used in molecular biology to synthesize complementary DNA (cDNA) from RNA by using a random primer instead of a specific primer sequence. This random primer initiates the synthesis at various sites along the RNA molecule, resulting in a mix of cDNA fragments for subsequent analysis
How to use "random priming" in a sentence
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random priming
Random priming was used to produce the cDNA.
All probes were labeled by random priming.
Random priming with hexanucleotide primers is used for reverse transcription reaction.
Radiolabelling was by the random priming method.
Random priming does not offer this possibility, the repair system only hardly offers it.
Specific probes for hybridization were made by random priming.
Another preferred method is random priming with mixed sequence primers followed by polymerase extension.
The fragment was labeled with radioactive nucleotides by random priming.
The non-PCR-based method may comprise random priming by a strand displacement polymerase.
The resulting fragment was gel purified and labeled by random priming.
Biotinylation of probes was achieved by random priming ( Invitrogen ) the four symbols separately.
These separated fragments were labeled with radioactive nucleotides by random priming.
The probe was radiolabeled using the random priming technique described in Example 2.
Second strand cDNA synthesis was performed with RNAse H treatment followed by random priming.
In DOP-PCR and variations, random priming is used to amplify the original material DNA.
See also
CDNA probes were labelled by random priming.
The probe was labeled with 32P by random priming using a kit obtained from Boehringer Mannheim.
Essentially, double-stranded cDNA was synthesized by oligo dT and random priming.
CDNA was synthesized from total RNA using random priming and Superscript II ( Invitrogen ) reverse transcriptase.
The probes ( cosmids ) are labeled by incorporation of a biotinylated nucleotide by the random priming technique.
The probes were labeled with 32P via random priming using the MegaPrime ™ system Amersham Biosciences, Cat.
Methods for effecting artificial gene recombination ( DNA shuffling ) include random priming recombination Z.
Probes were prepared using random priming kits ( Boehringer Mannheim, Indianapolis, IN ).
Radiolabeled 32P probes were prepared by the random priming method.
Labeling was performed by random priming using Ready-to Go DNA labeling beads ( Pharmacia ).
Both probes were labelled with 32P by hexamer random priming.
The probes were labeled by random priming using biotin-16-dUTP.
Triturus marmoratus, cytogenetics, heterochromatin, restriction endonuclease DNA denaturation, random priming.
The probe was labelled using a random priming kit ( Amersham ).
FIG . 31 depicts a method of stochastically labeling one or more molecules by random priming.
The fragment was labelled with Alpha 32P by random priming using the Promega Prime-a-Gene ® system.
Probe - Full length Hpa2 cDNA radiolabeled by random priming.
This coding region probe is P32 labeled by random priming ( Feinberg and Vogelstein ).
The blot was hybridized using a 1.2 kb Sma fragment labeled with ³ ² P using random priming.
The probe was radioactively labeled with 32P by random priming as disclosed above.
The filters were hybridized overnight at 68°C with DNA fragments labelled with 32P by random priming.
Northern Blot analysis The probes were labelled by random priming with dCTP - ( a ³ ² P ).
The PCR generated fragments were 32P-labelled using random priming.
Radiolabeled 32P-probes were prepared by the random priming method.
The fragment was radioactively labelled with 32P using the random priming method.
Probes were made from gel-purified DNA fragments and labeled by random priming ( Amersham ).
T4 DNA Polymerase, heat stable polymerases, Exo III Nuclease, random priming e . g.
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Faster priming due to improved flow technology
Conscious and unconscious priming paradigms are employed
Priming for starburst is making everything stop
Examples of using Random
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Banditry and random violence are on the increase
They just pick up any dish at random
Random physical inventories are performed on a monthly basis
