Examples of 'recombinant phage' in a sentence
Meaning of "recombinant phage"
recombinant phage: This term refers to a genetically engineered virus that infects bacteria. It is commonly used in molecular biology and genetic research
How to use "recombinant phage" in a sentence
Basic
Advanced
recombinant phage
One recombinant phage is isolated as positive clone.
A host cell is transformed with the recombinant phage or plasmid thus obtained.
This recombinant phage was cultured on agarose plates to make a λgtll cDNA library.
ScFv gene was constructed by recombinant phage display.
Purified recombinant phage enzymes can be used as separate antibacterial agents in their own right.
Thus, generally each virus will be a phage, and in particular a recombinant phage.
A fragment of the recombinant phage which contains the cDNA is subcloned into the bluescript plasmid.
Mouse anti human L-SIGN antibodies were identified using recombinant phage technology.
Specific binding of recombinant phage expressing this scFv was demonstrated by ELISA.
Collectively, the cDMA libraries contained 14 million primary recombinant phage.
One recombinant phage reactive with each Mab was ultimately identified.
Single-stranded DNA templates were prepared from recombinant phage DNA by polyethylene glycol treatment.
Recombinant phage plaques were identified using PCR.
A cDNA library consisting of approximately 1 x 10 ⁷ independent recombinant phage clones is generated.
The recombinant phage was packaged into infectious particles which were used to infect E.
See also
Collectively, the cDNA libraries contained 14 million primary recombinant phage.
In preferred embodiments, a recombinant phage receptor is produced by a recombinant bacterial cell.
Two cDNA libraries, each consisting of approximately 1 x 10 ⁷ independent recombinant phage clones were generated.
A recombinant phage containing a 15kb insert was identified and used for further analysis.
Coli is then infected with the recombinant phage ( eosi-cDNA library ).
The amount of recombinant phage bound was determined was determined as described in Example 3.
Example 4, Generation and isolation of Lys minus recombinant phage.
After recombinant phage is selected, the phage is infected in E. coli and multiplied.
Subsequently 100 µL of supernatant containing recombinant phage particles were added to pre-coated microtiter plate wells.
Recombinant phage DNA including both λ gt 10 and the cDNA was constructed as follows.
Preparation of recombinant phage DNA,.
These recombinant phage particles were verified for the expression of r-K2S by sandwich ELISA.
Only one clone in 2 x 106 screened recombinant phage was detected.
The desired recombinant phage is then selected and used to lysogenize a host strain of E. coli.
Two independent clones were isolated out of 4x105 recombinant phage plaques screened.
A positively-hybridizing recombinant phage is selected and DNA prepared using standard procedures.
Example 3, Creation of plasmid pGMB021 for use in Lys minus recombinant phage generation.
In the recombinant phage particles, the DNA inserts become fused to the gene for B-galactosidase.
Figure 12, Schematic representation and partial restriction map of the 6.3 kb insert of recombinant phage.
Recombinant phage were digested with EcoRI and the inserts subcloned into M13 for sequence analysis.
The resulting recombinant phage was named 31.
Recombinant phage DNA is encapsidated using Gigapack-plus kit from Stratagene.
The construct was delivered to the chromosome using a recombinant phage called AGebg-LacIQ 5 into F'tet /GM101.
Recombinant phage clones ( white placques ) were picked and the ends sequenced.
Each ligation reaction yielded approximately 2 X 10 ⁵ recombinant phage.
The resultant recombinant phage was designated mCa68.
Recombinant phage DNA including both λ gt 10 and cDNA was prepared as follows.
From each phage library 5 X 105 recombinant phage were obtained.
The recombinant phage stocks can be stored at 4 °C.
In this manner, 42 recombinant phage were selected and isolated and their DNA prepared.
Recombinant phage DNA from four positive plaques was purified, and each contained a 0.6 kb insert.
Approximately 2 x 105 recombinant phage are screened using total rabbit Taq polymerase antiserum.
Recombinant phage expressing HMW1 or HMW2 were recovered as follows.
The DNA from this recombinant phage EMBL4/A-3 clone was extracted and digested with EcoRl.
Recombinant phage antibody particles were produced using constructs 1-4 and analysed in an ELISA ( Figure 5 ).
You'll also be interested in:
Examples of using Recombinant
Show more
With recombinant human erythropoietin and controls
The active substance is recombinant salmon calcitonin
These recombinant types are expected to differ in stability
Examples of using Phage
Show more
The phage is covered by a protective protein coat
This strain of the phage is particularly virulent
Phage cocktails are sold in pharmacies in eastern countries
