Examples of 'renilla luciferase' in a sentence
Meaning of "renilla luciferase"
renilla luciferase: Renilla luciferase is a type of enzyme that produces light in the presence of its substrate coelenterazine. It is often used as a reporter gene in biological research to monitor gene expression, protein-protein interactions, and other cellular processes
How to use "renilla luciferase" in a sentence
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renilla luciferase
Renilla luciferase activity was normalized by firefly luciferase expression levels.
Transcriptional activation values are represented as firefly luciferase normalized to renilla luciferase.
Renilla luciferase expression serves as a control for transfection efficiency and cell viability.
Data for firefly luciferase was normalised to renilla luciferase to give a relative luciferase activity.
Luciferase activities were expressed as ratio between luciferase unit and renilla luciferase unit.
For the viruses containing the renilla luciferase gene no obvious additional species were observed.
The measured values were represented as values standardized by using the renilla luciferase activity.
Both renilla luciferase activity and firefly luciferase activity could be detected in individual plaques.
Firefly luciferase activity was normalized using renilla luciferase activity to correct for transfection efficiency.
The values of firefly luciferase were normalized to the values of Renilla luciferase.
Data are normalized to Renilla luciferase activity.
Cells were transiently or stably transfected with a plasmid encoding Renilla luciferase.
Renilla luciferase luminescence was normalized to total protein absorbance using BCA measuring assay.
Luciferase activities were normalized to Renilla luciferase values.
The amount of renilla luciferase activity was measured using a Pakcard TopCount NXT scintillation counter.
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The final results were normalized to Renilla luciferase activity.
Measuring the decrease in Renilla luciferase activity thus provides a convenient way of monitoring activity.
Efficiency of transfection was normalised using Renilla luciferase activity.
Renilla luciferase activity was measured in vivo using bioluminescence imaging after 8 d.
All relative luciferase activities were normalized to Renilla luciferase activity.
Firefly renilla luciferase signal pathway was measured by the dual-luciferase reporter gene test system.
Transfection efficiency was normalised with the Renilla luciferase activity.
For example, Renilla luciferase and protected luminophore may be added to a sample.
The firefly luciferase activity was evaluated and normalized by Renilla luciferase.
Figure 6 is a map of a Renilla luciferase expression plasmid.
The results are expressed as ratio of firefly luciferase activity to Renilla luciferase activity.
The CMC-driven Renilla luciferase was added to correct for transfection efficiency.
Each LUC activity was calibrated with the control Renilla luciferase activity.
Accordingly, Renilla luciferase is a potentially useful label for biological and other assays.
Firefly Luciferase activity was normalised for Renilla Luciferase activity per well.
Again, the synthetic Renilla luciferase gene RNA produced more luciferase than the native one.
As an internal control for transfection efficiency a constitutive renilla luciferase reporter ( promega ) was cotransfected.
The version of Renilla luciferase ( Rluc ) which incorporates all these changes is Rlucver1.
Advantageously, the energy donor protein is Renilla luciferase.
Purification of recombinant Renilla luciferase has allowed us to determine its amino-terminal sequence.
In preferred embodiments, a protected luminophore is used in an assay comprising Renilla luciferase.
Growth curves of different viruses having the renilla luciferase gene as compared to MHV-WT.
Relative repression of firefly luciferase expression standardized to a transfection control, Renilla luciferase.
As a result, there has been no development of Renilla luciferase as a detectable label.
Table 4 summarizes the affect of these selected chemicals on the luminescent activity of Renilla luciferase.
Figure 3 shows a complete recombinant Renilla luciferase amino acid sequence as produced by an expression system.
Figure 5 is a schematic representation of a restriction enzyme map for Renilla luciferase cDNA.
B, Original image of the light produced by the renilla luciferase oxidation of its substrate coelenterazine.
The lower portion of Figure 5 is a schematic representation of sequencing strategy for Renilla luciferase cDNA.
In preferred embodiments, the donor is Renilla luciferase and the acceptor is green fluorescent protein.
The relative repression of firefly luciferase expression ( Fold Change ) standardized to a renilla luciferase control.
Results were expressed as relative renilla luciferase activity ( renilla luciferase / firefly luciferase ).
This construct, pTZRLuc-1, was used in the expression and purification of recombinant Renilla luciferase.
An appropriate plasmid for this transfection is the Renilla luciferase plasmid available from Promega, no.
Firefly and renilla luciferase were measured 48 h after transfection using a Dual Luciferase Assay ( Promega ).
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Examples of using Luciferase
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No luciferase expression is observed in this control assay
Injection was measured by luciferase reporter gene expression
Luciferase determination was performed using a chemiluminiscence assay
