Examples of 'reporter protein' in a sentence
Meaning of "reporter protein"
reporter protein: In biology, this phrase typically refers to a type of protein that is used to track or monitor the activity of specific genes or cellular processes. Reporter proteins are often engineered to produce a detectable signal when they are expressed within a cell
How to use "reporter protein" in a sentence
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reporter protein
The polynucleotide can encode a reporter protein.
As reporter protein.
These methods are based on a fusión of a reporter protein to the target protein.
The reporter protein provides a detectable means for evaluating gene expression.
Suitable techniques for detecting reporter protein activity in cells are known in the art.
A reporter protein may be a naturally occurring protein or a protein that is not naturally occurring.
Fusion of the recombinant proteins to a reporter protein permits simple determination of its solubility.
Reporter protein activity may be measured using an assay specific to the reporter being used.
The cell usually comprises a reporter gene that encodes the reporter protein.
Those progeny fish not expressing the reporter protein can be excluded from further analysis.
The efficacy of the candidate may be evaluated by its effect on reporter protein activity.
Thus, the reporter protein is thermostable.
Monitoring the expression of the light emitting reporter protein from the cell.
The reporter protein is preferably a stable variant of beta-galactosidase.
The resulting reporter gene should then express fully active reporter protein.
See also
In an alternative embodiment, the reporter protein is an auxotrophy marker.
This treatment activates the luciferase gene and causes the production of the luciferase reporter protein.
Thus, reporter protein expression is linked to activation of the receptor.
Sections are stained with hematoxylin and eosin and processed for fluorescent reporter protein microscopy.
In a preferred embodiment, the reporter protein is a protein conferring resistance to an antibiotic.
It is also preferred that the DNA sequence codes for a reporter protein.
In a more preferred embodiment, the reporter protein is a thermostable kanamycin nucleotidyltransferase.
In some cases, the transgene encodes a reporter protein.
Preferably, the reporter protein is a thermostable version of a kanamycin or bleomycin resistance protein.
GFP has been used as reporter protein.
In reporter protein assays, a protein which is sensitive to desired events is expressed in a cell.
In some embodiments, the transgene encodes a reporter protein.
The method of claim 4, wherein the reporter protein is a green fluorescent protein.
Kringle 3 domain of human plasminogen was used as the reporter protein.
Alternatively, the reporter protein can be a protein exhibiting natural fluorescent properties e . g.
Then, each cell contains a comparable amount of reporter protein.
Further, the reporter protein is preferably a fluorescent protein, more preferably a green fluorescent protein.
Typically, such antigens do not qualify as selection, marker or reporter protein.
After transformation, the expression of the reporter protein on the membrane was examined.
The transgenic zebrafish of claim 4 wherein the expression product is a reporter protein.
The transgenic zebrafish of claim 6 wherein the reporter protein is green fluorescent protein.
The method of claim 1, wherein the expression product is a directly detectable reporter protein.
The process according to claim 1, wherein the thermostable reporter protein is a thermostable kanamycin nucleotidyltransferase.
In a particular embodiment, the phenotype may be assessed by evaluating the production of a reporter protein.
This system comprises cephalosporinase as a reporter protein anchored on a cell wall of gram-positive bacterium.
Once activated, this system would produce extremely high levels of the reporter protein.
For example, a reporter protein may be a fusión protein having an epitope tag fused to a protein.
Green fluorescence protein ( eGFP ) was used as intracellular reporter protein for promoter activity.
A reporter protein is expressed with a His tag, purified and then analyzed.
For example, after a cross, progeny fish can be screened for expression of the reporter protein.
A preferred reporter protein that can be directly detected is the green fluorescent protein ( GFP ).
In another embodiment, the mammalian expression vector further comprises a nucleic acid sequence encoding a reporter protein.
Also, the present invention includes Tf fused to a reporter protein or to a detectable protein.
Preferably, the reporter protein is GFP ( green fluorescent protein ) or a variant of GFP.
Table 5, Comparison of the short and extended Pchilpromoters in terms of reporter protein expression level.
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