Examples of 'restriction enzyme digestion' in a sentence
Meaning of "restriction enzyme digestion"
restriction enzyme digestion: A technique commonly used in molecular biology to cut DNA into smaller fragments. This process is crucial in genetic engineering and DNA analysis
How to use "restriction enzyme digestion" in a sentence
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restriction enzyme digestion
No restriction enzyme digestion or specific primers are required.
Recombinant plasmids were confirmed restriction enzyme digestion.
Using restriction enzyme digestion.
The recombinant plasmids were verified by restriction enzyme digestion.
Clones were analyzed by restriction enzyme digestion for the presence of an insert.
The linearization of pBC was done by a single restriction enzyme digestion.
Clones yielding an appropriate restriction enzyme digestion pattern were sequenced to confirm the mutation.
The structure of the plasmid was confirmed by restriction enzyme digestion.
Restriction enzyme digestion was performed in accordance with the manual of each restriction enzyme.
Mutants were screened by restriction enzyme digestion.
Restriction enzyme digestion analysis was performed to confirm the authenticity of each ligated fragment.
Clones were analyzed by restriction enzyme digestion with PacI.
Correct orientation of the cloned HindIII fragment was confirmed by restriction enzyme digestion.
Mutagenesis was checked by restriction enzyme digestion and sequencing.
Plasmids were isolated from kanamycin resistant clones and checked by restriction enzyme digestion.
See also
restriction enzyme analysis
restriction enzyme cleavage
restriction enzyme map
restriction enzyme sites
restriction enzyme cleavage
restriction enzyme map
restriction enzyme sites
Plasmids have to be linearized by restriction enzyme digestion before transfections.
The correct orientation of the subcloned HindIII fragment was determined by restriction enzyme digestion.
The sequences were confirmed by restriction enzyme digestion and sequencing.
Plasmids were isolated from the ampicillin resistant colonies and analyzed by restriction enzyme digestion.
Recombinant clones were analyzed by restriction enzyme digestion to determine insert orientation.
The orientation of the IT transcriptional control region was confirmed by restriction enzyme digestion.
Positive colonies were identified via restriction enzyme digestion and sequencing reactions.
Or, restriction enzyme digestion of unwanted fragments can be used to prevent ligation.
Positive colonies were identified via restriction enzyme digestion.
Perform restriction enzyme digestion analysis of the miniprep DNA.
The constructs were confirmed vía restriction enzyme digestion and sequencing.
Alternatively, restriction enzyme digestion of unwanted fragments can be used to prevent ligation.
The orientation of the enhancer was confirmed by restriction enzyme digestion.
Transformants were analyzed by restriction enzyme digestion and Southern hybridization analysis.
Plasmids with correct insertion were analyzed by restriction enzyme digestion.
DNA fragments after restriction enzyme digestion were separated by agarose gel electrophoresis.
The orientation of the fragment was confirmed by restriction enzyme digestion.
Restriction enzyme digestion for the allele exchange plasmid pAF102.
The construct was confirmed vía restriction enzyme digestion and sequencing.
Preferably, the vector, preferably the circular vector, is linearizable, for example, by restriction enzyme digestion.
The transformants were verified by restriction enzyme digestion and one was frozen as a stock.
Other mutations were analyzed by the PCR technique in association with restriction enzyme digestion.
JE sequences were verified by restriction enzyme digestion and nucleotide sequencing.
Approximately one microgram of the viral DNA is then submitted to restriction enzyme digestion.
This step is needed to facilitate restriction enzyme digestion of PCR fragment for future ligation.
The toxin gene can be excised from the plasmid DNA by restriction enzyme digestion.
Phage GΦ5 was mapped by restriction enzyme digestion and DNA sequencing.
A clone was identified which contained a plasmid with the expected DNA restriction enzyme digestion pattern.
DNA fragments obtained by the restriction enzyme digestion were subjected to electrophoresis using 0.7 % agarose gel.
Correct orientation of the gene was confirmed by restriction enzyme digestion.
Key words, aneuploidy, atrazine, restriction enzyme digestion chromosome banding, Pacific oyster, Crassostrea gigas.
The other seven BACs were fingerprinted by restriction enzyme digestion.
Restriction enzyme digestion proceeded at 37°C for 3 hours using restriction enzyme buffer 4.
The expected sequence was confirmed by restriction enzyme digestion and sequencing.
Hybridising clones were purified, DNA was isolated from each and mapped by restriction enzyme digestion.
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