Examples of 'restriction fragment' in a sentence
Meaning of "restriction fragment"
A restriction fragment is a DNA fragment that is produced by cutting a DNA molecule at specific recognition sites using restriction enzymes. This term is primarily used in the field of molecular biology to describe the fragments resulting from the enzymatic digestion of DNA for various scientific experiments or genetic studies
How to use "restriction fragment" in a sentence
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restriction fragment
Assessing the restriction fragment pattern by electrophoresis.
A phylogenetic tree was assembled from mitochondrial restriction fragment data.
Clones were screened by restriction fragment analysis and sequenced.
Restriction fragment length polymorphism.
The expected amplicon and restriction fragment are present.
The restriction fragment containing the replication origin was purified.
All plasmid constructions are confirmed by restriction fragment analysis and sequencing.
The restriction fragment was purified on an agarose gel and eluted.
The portion of the gel containing this restriction fragment was recovered.
Use of restriction fragment length polymorphism to distinguish between salmon species.
Certain hybrid gene constructions could not be accomplished by restriction fragment subcloning.
Restriction fragment length polymorphism assay for detection of homologous recombination.
Undesired nucleotides in the restriction fragment may optionally be removed by standard methods.
Restriction fragment length polymorphism analysis demonstrated this classification scheme needed revision.
The expected size of each restriction fragment is indicated above the line.
See also
There are two common mechanisms by which the size of a particular restriction fragment can vary.
This restriction fragment is now ready to be inserted in an appropriate expression vector.
AFIP is a method for selective restriction fragment amplification.
The shorter restriction fragment can then be inserted into a suitable vector.
DNAs are compared by looking for differences in restriction fragment lengths.
Clones were screened for the restriction fragment and candidates were sent for sequencing.
Restriction fragment length differences were compared in Philonema spp.
The latter example is called restriction fragment length polymorphism RFLP.
Haemagglutination positive clones were selected and checked by DNA restriction fragment analysis.
An authentic BamHI restriction fragment was generated by digesting this litigation mixture with BamHI.
This PCR amplification allows detection of restriction fragment length polymorphisms.
The immediate early promoter of the human cytomegalovirus is conveniently obtained as a HindIII E restriction fragment.
Subsequent analysis was by restriction fragment length polymorphism and DNA sequencing.
The immediate early promoter of the human cytomegalovirus is conveniently obtained as a HindIll E restriction fragment.
Investigation will include the study of restriction fragment length polymorphism RFLP.
RFLP Restriction fragment length polymorphism is a technique used to detect variations in homologous DNA.
A Southern analysis identifies two distinct restriction fragment length polymorphisms in the human population.
We digested the PCR product with AatII and isolated the resulting restriction fragment.
Isolation of a restriction fragment encoding modified lipase:.
A positive identification can be made with molecular analyses such as restriction fragment length polymorphism RFLP.
The principle of multiplex restriction fragment amplification is illustrated in figure 4.
Viral DNA was detected by Southern blot analysis with a radioactively adenoviral restriction fragment.
Preferably, the polymorphism is a restriction fragment length polymorphism.
A cDNA encoding the complete Kbsol protein could be obtained as a Bam HI restriction fragment.
Thus, SNPs are rarely detectable restriction fragment length analysis.
The immediate early promoter of the human cytomegalovirus is conveniently obtained as a Hindlll E restriction fragment.
Thus, SNPs are sometimes detectable by restriction fragment length analysis.
Figure 2 shows the restriction fragment band pattern on an electrophoresis gel belonging to this experiment.
This results in a set of contigs, each corresponding to a particular restriction fragment.
However, detection by sequencing of the entire restriction fragment is still relatively uneconomical.
The sequence is determined as outlined, including the identifier sequence and the restriction fragment.
Primers, kits and sets of restriction fragments used in selective restriction fragment amplification.
The adaptor is ligated ( covalently connected ) with one or both sides of the restriction fragment.
A restriction fragment containing the above-mentioned functional elements was then transferred into an expression vector.
Genotyping was performed by polymerase chain reaction - restriction fragment length polymorphism pcr-rflp.
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Examples of using Fragment
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He has a fragment of sword embedded in his chest
There is a tiny missing fragment on the occipital
A small fragment impinging on her spinal cord
