Examples of 'restriction mapping' in a sentence
Meaning of "restriction mapping"
Restriction mapping refers to the process of mapping or identifying the locations and specific sequences of restriction sites (specific DNA sequences recognized and cut by restriction enzymes) within a DNA molecule. This technique is used in molecular biology to analyze and study the structure of DNA, particularly in determining the order of DNA fragments or the location of specific genes within a DNA sequence
How to use "restriction mapping" in a sentence
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restriction mapping
These results were further verified by restriction mapping.
Restriction mapping requires the use of restriction enzymes.
The constructs were analyzed by restriction mapping and sequenced.
Restriction mapping revealed several convenient sites for subcloning.
The plasmids were examined by restriction mapping.
Then do miniprep and restriction mapping to screen for desired product.
The structure of the plasmid was verified by restriction mapping.
The clone was analysed by restriction mapping and a preliminary restriction map was constructed.
The orientation of the cDNAs was confirmed by restriction mapping.
Clonal isolates were chosen by restriction mapping and verified by sequencing through the linker.
Further confirmation was performed by restriction mapping.
Additional restriction mapping indicated a unique BssHII site at one end of the viral genome.
Plasmid DNAs were obtained and analysed by restriction mapping.
Restriction mapping allows scientists to distinguish between the DNA of different individuals.
Individual clones were analysed by restriction mapping.
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Detailed restriction mapping coupled with DNA sequencing confirmed this assumption.
Several clones were subsequently identified and analyzed by restriction mapping.
All the constructs were verified by restriction mapping and nucleotide sequencing.
These clones were isolated and further characterized by restriction mapping.
Mutant plasmids were selected by restriction mapping the resulting PCR amplicons.
Presence of the plasmid is confirmed by antibiotic selection and restriction mapping.
Positive clones were then characterized by restriction mapping to identify groups of clones.
Clones with the insert in the correct orientation were identified by restriction mapping.
The resulting constructs are characterized by restriction mapping and confirmed by dsDNA sequencing.
Positive clones isolated from the screen were characterized by restriction mapping.
The constructed gene was verified by restriction mapping and DNA sequence analysis.
The plasmid DNAs of these clones were further analyzed by restriction mapping.
Restriction mapping and Southern blot hybridization analysis were used to confirm this finding.
Insertion was verified by restriction mapping.
Restriction mapping identified a common and unique internal Sph i site in both FIV genomes.
The resulting plasmid was confirmed by restriction mapping and sequencing.
The basis to restriction mapping involves digesting ( or cutting ) DNA with restriction enzymes.
The recombinant clones were further characterized by restriction mapping and sequencing.
Six clones are isolated ; restriction mapping indicates that they fall into two gene classes.
Colonies were grown and mini plasmid preps were analyzed by restriction mapping.
This virus was characterized by restriction mapping and the SOUTHERN BLOTTING DNA procedure.
Those colonies positive with both probes were then analyzed by restriction mapping.
The insert was checked by restriction mapping and dideoxy-sequencing.
The clone containing sequence in the antisense orientation was determined by restriction mapping.
These DNA fragments are characterized by restriction mapping and sequence analysis.
The structure of the final plasmids were verified by nucleotide sequencing and restriction mapping.
The correctness of the constructions is confirmed by restriction mapping and direct DNA sequence determination.
The orientation of STAR elements in recombinant pSDH vectors was determined by restriction mapping.
The ligation was confirmed by restriction mapping after transforming the constructs into DH5a.
Correct ligation and orientation of each construct were checked by restriction mapping and sequencing.
DNA sequence analysis and restriction mapping confirmed the authenicity of the t-PA cDNA.
Transformants were isolated and plasmids were prepared and analysed by restriction mapping and sequencing.
Restriction mapping and fragment isolation were performed with LiCl ₄ extracted plasmid DNA.
Their structure was carefully verified during and after construction by restriction mapping and sequencing.
Restriction mapping of recombinants revealed those containing a single B-ketothiolase insert in the sense orientation.
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