Examples of 'reverse transcriptase activity' in a sentence
Meaning of "reverse transcriptase activity"
Reverse transcriptase activity: Refers to the ability of the enzyme reverse transcriptase to generate complementary DNA from an RNA template. It is commonly used in molecular biology techniques like reverse transcription polymerase chain reaction (RT-PCR) to study gene expression
How to use "reverse transcriptase activity" in a sentence
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reverse transcriptase activity
Because we found reverse transcriptase activity.
The reverse transcriptase activity of the compounds of the invention may be assayed as following.
An enzyme having a reverse transcriptase activity.
Radioactive incorporation into trichloroacetic acidprecipitable nucleic acids indicated the presence of reverse transcriptase activity.
Tth polymerase also has reverse transcriptase activity.
The specific reverse transcriptase activity of the pools was determined by a radioactive test system.
Aliquots of the lysate were assayed for reverse transcriptase activity.
Reverse transcriptase activity can be quantitated using commercially available kits and following the manufacturers protocol.
Effects on virion production and reverse transcriptase activity are then determined.
The reverse transcriptase activity described provides a cDNA transcript from an RNA template.
Reverse transcriptases include any enzyme having reverse transcriptase activity.
An enzyme having reverse transcriptase activity and optional Rnase H activity.
The samples were normalized prior to assay using reverse transcriptase activity.
Optimized reverse transcriptase activity assay,.
The term encompasses polymerases that have reverse transcriptase activity.
See also
reverse transcribed
reverse transcriptase
reverse transcriptase inhibitor
reverse transcriptase inhibitors
If reverse transcriptase activity is present, it confirms that the virus is a retrovirus.
The enzyme possesses efficient reverse transcriptase activity in the presence of manganese.
Also preferred is that the kit contains an enzyme with reverse transcriptase activity.
In another version, the reverse transcriptase activity is a component of the thermostable Tth DNA polymerase.
Significant amounts of RNAse activity eluted with the reverse transcriptase activity.
Reverse Transcriptase Assay, Reverse transcriptase activity was measured in cell-free supernatants.
HIV infection can be followed by syncitium formation or reverse transcriptase activity.
But Gallo had already found reverse transcriptase activity in the leukemia cells.
The RT sequence may encode a mutation to substantially inactivate any reverse transcriptase activity.
Virus production was monitored by the reverse transcriptase activity as well as antigen capturing.
The nucleocapsid encloses the viral DNA and a DNA polymerase that has reverse transcriptase activity.
The y-axis represents reverse transcriptase activity in cpm.
A purified DNA polymerase according to claim 1 or 2 which has reverse transcriptase activity.
A DNA polymerase comprising reverse transcriptase activity ( Reverse Transcriptase ).
For example, at least one of the polymerases may comprise reverse transcriptase activity.
The reverse transcriptase activity will then extend the sequence from the hybridized 3 ' - hydroxyl terminus.
The production of virus was determined by following reverse transcriptase activity over time.
Furthermore, the reverse transcriptase activity is reduced by 90 % in said clone.
The particles produced by this method had protease and reverse transcriptase activity and were infectious.
Optimal reverse transcriptase activity is observed between 50 and 75 mM KCl.
The pellet was decanted and analyzed for reverse transcriptase activity as follows.
The reverse transcriptase activity in the virus-infected control was about 2 x 105 dpm per ml.
Viral replication was measured by assaying the reverse transcriptase activity in the culture supernatants.
The virus has a diameter of approximately 130 nm . the virus possesses a magnesium dependent reverse transcriptase activity.
Table 6 gives the results obtained for reverse transcriptase activity with the control samples.
Inhibition of viral protease activity . Binding to and inhibition of the viral reverse transcriptase activity.
Culture supernatant was assayed for reverse transcriptase activity 8 days later ( Table 6 ).
The method of paragraph 57, wherein at least one of said polymerases comprises reverse transcriptase activity.
All cultures were maintained and monitored for reverse transcriptase activity for 6 weeks before being discarded.
In the early 1980s, Barré-Sinoussi was already familiar with the technique of detecting reverse transcriptase activity.
The tested compounds did not inhibit HIV reverse transcriptase activity at a concentration of 100 µg / µL.
The thermostable DNA polymerase enzyme as claimed in claim 1, which has reverse transcriptase activity.
Ever since, reverse transcriptase activity has been used as a “marker” for HIV….
This experiment demonstrates that Taq has reverse transcriptase activity.
The graph shows reverse transcriptase activity ( 32P incorporated ; cpm ) for sucrose gradient fractions.
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