Examples of 'reverse transcription reaction' in a sentence
Meaning of "reverse transcription reaction"
reverse transcription reaction: A scientific process involving the conversion of RNA into DNA, typically encountered in molecular biology and genetics
How to use "reverse transcription reaction" in a sentence
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reverse transcription reaction
This step is a standard reverse transcription reaction.
The reverse transcription reaction was carried out as follows.
Shown are the products of a reverse transcription reaction.
Reverse transcription reaction was performed as follows.
An optional amplification reaction or the reverse transcription reaction.
A mock reverse transcription reaction was used as a negative control.
An improved polymerase of the invention may be used in a reverse transcription reaction.
The reverse transcription reaction may be performed separately from the amplification reaction.
Two reaction mixes for the reverse transcription reaction are prepared.
Serial dilutions were made with the pool of cDNA obtained from the reverse transcription reaction.
The product from the reverse transcription reaction is amplified in two separate reactions.
The washed tube was immediately subjected to the reverse transcription reaction.
The reverse transcription reaction products are suitable as templates for amplification by PCR.
A reverse transcriptase can be employed to carry out the reverse transcription reaction.
CDNA produced by a reverse transcription reaction using RNA as a template is also included.
See also
Random priming with hexanucleotide primers is used for reverse transcription reaction.
The product from the reverse transcription reaction is amplified in a second PCR reaction.
PCR reactions were carried out on the reverse transcription reaction.
The reverse transcription reaction was performed using the RevertAid First Strand cDNA Synthesis kit.
PCR was carried out from cDNAs obtained by reverse transcription reaction cf.
The reverse transcription reaction was performed as follows, µg.
The operation is performed with two steps of reverse transcription reaction and PCR.
A reverse transcription reaction can be conducted in a presence of a 3 ' tagging polynucleotide.
Total RNA extracted from the cell was used to synthesize cDNA by reverse transcription reaction.
The generated RNA is subsequently used for a reverse transcription reaction using retrotransposon specific reverse transcriptase.
In some embodiments, an improved polymerase of the invention is used in a reverse transcription reaction.
First, reverse transcription reaction was conducted in the following reaction mixture.
CDNA first strand was synthesized from total RNA by reverse transcription reaction.
Amplifying products of the reverse transcription reaction to produce amplification products ; and.
Using the total RNA as a template, cDNA was synthesized by reverse transcription reaction.
In some embodiments, a single reverse transcription reaction is sufficient to assay multiple target RNAs.
Initially, mRNA was copied into complementary DNA in a conventional reverse transcription reaction.
A reverse transcription reaction was carried out with reverse transcriptase following the manufacturer 's instructions.
Single-stranded cDNAs resulting from the reverse transcription reaction were spotted on the membrane.
Two reverse transcription reaction mixes were prepared with as described in Example 1 above.
Subsequently, the extension primer is extended in a reverse transcription reaction using the miR as template.
The reverse transcription reaction mixture is then placed on ice in preparation for PCR . iv.
For PCR, 5 µl of cDNA directly from the reverse transcription reaction was amplified.
In some cases, a reverse transcription reaction and a PCR reaction are carried out in a different droplet.
Dispense 80 µl of the PCR master mix into each reverse transcription reaction tube.
In other embodiments, the reverse transcription reaction is carried out with a suitable thermoactive DNA polymerase.
As a consequence, the cellular RNA may become available for the reverse transcription reaction.
Performing a reverse transcription reaction according to the method of claim 1 ;.
After one hour, total RNA was extracted and subjected to the reverse transcription reaction.
The total reverse transcription reaction volume should be 20 µl per sample.
Collected Dynabeads with immobilizes elongated mRNA-dsDNA complex was resuspended in reverse transcription reaction mixture.
The reverse transcription reaction typically is primed with specific primers, random hexamers, or oligo ( dT ) primers.
RNA from three sub clones of 2c3 was isolated and cDNA prepared by a reverse transcription reaction.
The enzyme used to carry out the reverse transcription reaction is SuperScript ™ III ( Invitrogen ).
The reverse transcription reaction and subsequent PCR amplification was performed with a 3 ' - RACE kit ( Gibco BRL ).
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