Examples of 'ribosome display' in a sentence
Meaning of "ribosome display"
ribosome display - This phrase refers to a laboratory technique used in molecular biology and biochemistry to study protein-RNA interactions. It involves the in vitro selection and amplification of proteins based on their binding affinity to ribosomal RNA. Ribosome display is commonly used in protein engineering and drug discovery research
How to use "ribosome display" in a sentence
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ribosome display
Protein of interest is displayed in ribosome display format.
Ribosome display is known in the art.
Polymerase of interest has to be displayed in ribosome display format.
Ribosome display selection rounds.
Protein of interest has to be displayed in ribosome display format.
Ribosome display offers the largest potential libraries and is fully in vitro.
These constraints limit the applicability of ribosome display selection.
In ribosome display the genetic material is replicated by enzymatic amplification with polymerases.
This allows for exponential replication of a ribosome display library in vivo.
Ribosome display is also useful for rapid large scale mutational analysis of antibodies.
Binding of the variable regions is mostly optimized by using ribosome display.
Ribosome display or mrna display method with selection for increased stability of the protein.
Such a construct generally comprises additional features for ribosome display.
Phage and ribosome display technologies for generating human antibodies are well known in the art.
This sample was used for the next ribosome display cycle.
See also
The RNA displayed in the ribosome display system is labile and prone to degradation.
The selection protocol differs from the usual in ribosome display.
Great care was taken during all ribosome display steps to avoid Rnase contamination.
To this end display techniques such as phage display or ribosome display are used.
The helix library was ligated to the Ribosome Display spacer using a restriction ligation procedure.
Selections for novel antibodies were performed using standard ribosome display protocols.
The Ribosome Display spacer was generated as follows.
The diversified clones are further screened in phage display or ribosome display libraries.
The ribosome display procedure was repeated 6 times.
Selections of binders against several different targets were successfully carried out using ribosome display.
Figure 2 illustrates a ribosome display construct according to an embodiment of the present invention,.
Methods of the invention incorporate a number of features not previously used together in ribosome display.
Encapsidated ribosome display RNA may also be co-translationally disassembled.
Nucleic acid could be also fused to a target through a translational process such as ribosome display.
Ribosome display selection rounds For selection experiments, biotinylated target proteins were used.
Selection of genes from a total mRNA library from mouse spleen cells by ribosome display.
The design of a ribosome display construct incorporating MDV and OAS sequences is shown in Figure 6.
Sensitive DNA recovery from mRNA is required in ribosome display.
Ribosome display and selection has the potential to generate and display large libraries ( 1014 ).
The OPG RNA library was then used for ribosome display.
In this context, they used molecular selection systems such as phage and ribosome display.
Materials and method of the arm ribosome display cycle ( figure 1 ).
Non-covalent linkage between phenotype and genotype can be achieved with ribosome display.
Figure 21, Schematic representation of a round of ribosome display selection.
After agarose gel analysis, the PCR product is ready for ribosome display.
FIG . 21, Schematic representation of a round of ribosome display selection.
Description of the figures Figure 1 . DNA construct for the generation of ribosome display complex.
The process of claim 3 or claim 4, wherein said selection step is performed by ribosome display.
One exemplary method includes screening protein expression libraries, e.g., phage or ribosome display libraries.
The plasmid pIVEX2.3MCSRD contained the DNA sequence of the ribosome display spacer.
Figure 7a schematic illustration of the single-chain A6 TCR-C-Kappa DNA ribosome display construct.
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