Examples of 'rnase-free water' in a sentence
Meaning of "rnase-free water"
Rnase-free water: Water that is free of ribonuclease (RNAse), an enzyme that can degrade RNA. Commonly used in molecular biology experiments to prevent RNA degradation and contamination of RNA samples
How to use "rnase-free water" in a sentence
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rnase-free water
The pellet was resuspended in RNase-free water.
Total RNA was eluted with RNase-free water and its concentration was determined by spectrophotometer.
Dissolve the pellet in the desired volume of RNase-free water or buffer.
Use RNase-free water and buffers.
In certain embodiments, eluting the captured mRNA involves recirculating the RNAse-free water.
Pipet 10 µl RNase-free water directly onto the spin column membrane.
The reaction was scaled by combining the RNA and RNase-free water.
Purified RNA shall be re-suspended in distilled RNase-free water namely water treated with 0,1 % diethyl pyrocarbonate.
The pellet was air dried and resuspended in 50 µl of RNase-free water.
Add 500 µL of DNAse Rnase-free water to the RNA pellet to dissolve it completely.
The pellet was resuspended in 3 µl RNase-free water.
A kit can comprise RNAse-free water.
In some embodiments, the eluting step comprises re-solubilizing the captured precipitated mRNA with RNAse-free water.
Total RNA was eluted with 48 µl of RNase-free water for each column.
After synthesis, the iRNAs are first of all taken up in RNAse-free water.
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RNA pellets were resuspended in RNase-free water ( Invitrogen ).
The elution step was repeated with a second volume of 50 µl RNase-free water.
Finally, the total RNA was eluted in RNase-free water.
After ethanol precipitation, the RNA pellet was dissolved in 50 µl sterile RNase-free water.
The RT reaction step was completed by adding 5.9µl of RNase-free water to a reaction tube.
After the last washing step, RNA was recovered from the columns in 50 µl RNAse-free water.
The total RNA 's were eluted with RNase-free water.
After the extraction step, a precipitate is resuspended in 25 µl RNase-free water.
In other of these embodiments, the initial sample comprises RNase-free water and / or TE buffer.
The sample was evaporated until dry, and dissolved in 20ul RNase-free water.
The cDNA was diluted 1 in 5 using RNase-free water.
For ethanol precipitation, adjust the volume of each sample to 180 µl using RNase-free water.
High-quality RNA was then eluted in RNase-free water.
For example, captured mRNA may be eluted with RNAse-free water.
The volume is adjusted to 70 µl with RNase-free water.
The RNA was eluted in 30 µl of RNase-free water.
Total RNA was eluted with 50 µL of RNase-free water.
The RNA was eluted using 40 µl RNAse-free water.
At last, the RNA was re-suspended in RNase-free water.
The mRNA was eluted with 50 µl Rnase-free water.
The pellet was dried and resuspended in 20 µl of RNase-free water ( Promega ).
After the RNA was washed with 70 % ethanol, it was dissolved in RNAse-free water.
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