Examples of 'rtta' in a sentence
Meaning of "rtta"
rtta (noun) - RTTA is an acronym that stands for Reverse Tetracycline Transactivator. It is a molecular biology tool used for gene expression studies
How to use "rtta" in a sentence
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rtta
Cell culture and rtTA activity assay.
Nucleotide and amino acid sequence of rtTA.
Mutations observed in rtTA can improve sc rtTA activity.
Characterization of the evolved rtTA variants.
Mutation of the rtTA gene through viral evolution.
All mutations enhanced sc rtTA activity.
Both the transactivators rtTA and sc rtTA are activated by doxycycline.
Combining the beneficial mutations further improves rtTA activity.
Mutations that enhance rtTA activity do not improve tTA activity.
Mutations do not affect the intracellular rtTA protein level.
The invention further provides rtTA and sc rtTA variants that are genetically stable.
It is therefore desired to improve the genetic stability of current rtTA systems.
Allows us to determine the level of rtTA activity that is required for viral replication.
Infected cell samples were used to analyze the proviral rtTA sequence.
The sc rtTA gene contains two TetR domains and a single activation domain.
See also
Our results demonstrate that all mutations did significantly enhance sc rtTA activity.
Said sc rtTA preferably comprises two TetR moieties and one eukaryotic regulatory domain.
These combinations further improve rtTA activity both at low and high dox levels.
Several viruses from independent cultures had acquired additional mutations in the rtTA gene.
Preferably rtTA and single chain rtTA variants are provided with at least one improved property.
Comparison of the rtTA activity data Fig.
RtTA and sc rtTA variants are provided that are inducible by antibiotics other than doxycycline.
Results Mutations observed in rtTA improve sc rtTA activity.
RtTA activity assay.
The luciferase level measured two days after transfection reflects rtTA activity Fig.
Sc rtTA is a single-chain version of rtTA.
Materials and Methods Construction of sc rtTA variants.
Novel rtTA variants can be activated by dox-like compounds.
These methods are non-limiting examples of generating sc rtTA.
Novel rtTA variants can be activated by dos-like compounds.
Amino acid substitutions in rtTA confer the loss of dox-control.
We subsequently determined the intracellular luciferase level, which reflects rtTA activity.
Construction of novel HIV-rtTA variants and rtTA expression plasmids.
In fact, these variants demonstrate a transcriptional activity and dox-sensitivity similar to rtTA.
We constructed rtTA expression plasmids with all possible amino acids at position 37.
The position and molecular weight ( in kDa ) of the rtTA and TetR proteins are indicated.
Said codon at rtTA amino acid position 56 preferably encodes a glutamine or a lysine residue.
In addition, the cells will be infected with a lentivirus vector transducing the rtTA transactivator.
Mutations in rtTA may improve rtTA function, and thus enhance viral replication.
Transcriptional activity and dox-sensitivity of wild type, naturally evolved and constructed rtTA variants.
The invention furthermore provides rtTA and sc rtTA variants with altered inducer-specificities.
In this Example, we tested whether these mutations similarly affect sc rtTA.
The activity of these rtTA variants was analyzed as described above for the position 37 variants.
Replication of such a virus is not dependent on a virally encoded transactivator, neither Tat nor rtTA.
Importantly, these rtTA variants do not show any basal activity in the absence of dox.
Testing all possible position 19 variants of rtTA.
Novel rtTA variants show increased activity and dox-sensitivity in different Tet systems.
Moreover, these mutations broaden the inducer-specificity of rtTA.
RtTA variants improve HIV-rtTA replication.
Transcriptional activity of rtTA variants with alternative amino acids at position 19 or 37.
